摘要
目的 :合成新型的复合生物材料框架作为骨组织工程研究的细胞外基质材料。方法 :本研究采用材料学自组装技术的原理 ,以I型胶原蛋白为分子模板 ,引导钙磷盐在液相中的矿化 ,制备具有天然骨基质层状结构的羟基磷灰石 /胶原复合材料 ,并以热致分相法制备了羟基磷灰石 /胶原 -聚乳酸复合三维多孔框架。结果 :羟基磷灰石 /胶原复合材料具有与天然骨基质相似的成分与结构 ,加入聚乳酸制备成三维多孔框架 ,孔隙直径界于5 0 μm— 30 0 μm。结论 :羟基磷灰石 /胶原 -聚乳酸复合三维多孔框架可能作为骨组织工程良好的细胞外基质材料。
To develop a novel complex biomaterial scaffold as extracellular matix for tissue engineering of bone, hydroxyapatite/collagen (HAC) sandwich composite was prepared to mimic the natural matrix of bone, with the collagen serving as a template for apatite formation. A three-dimensional highly porous scaffold was developed by mixing HAC with poly(L-lactic acid) (PLA) using a thermally induced phase separation technique (TIPS). Using type I collagen to template mineralization of calcium and phosphate in solution, the HA/collagen sandwich composite was obtained, mimicking the natural bone both in composition and in microstructure. The three-dimensional hydroxyapatite/collagen-Poly(L-lactic acid) (HAC-PLA)scaffold synthesized by TIPS has a high porosity of up to 90%, with, the pore size varying from 50μm to 300μm. The novel three-dimensional highly porous HAC-PLA scaffold is likely to be applied in tissue engineering of bone.
出处
《中国生物医学工程学报》
EI
CAS
CSCD
北大核心
2004年第5期438-442,397,共6页
Chinese Journal of Biomedical Engineering
基金
国家 8 6 3高技术研究发展计划 ( 715- 0 0 9- 0 510 )
关键词
羟基磷灰石
胶原
聚合物
组织工程
生物材料
Bone
Living systems studies
Porous materials
Synthesis (chemical)
