摘要
目的:探讨植物血凝素(PHA)诱发红细胞凝集反应及对凝血系统的影响。方法:12只Wistar大鼠随机分为实验组和对照组,分别经尾静脉注射植物血凝素和生理盐水,观察红细胞在体内外的凝集反应、溶血现象,大鼠的一般状况以及大鼠凝血指标的变化情况。结果:植物血凝素在体内外均能诱发凝集反应。实验组大鼠表现为竖毛、弓背、蜷缩、颤抖,而对照组大鼠无上述表现。实验组大鼠纤维蛋白原显著低于对照组,凝血酶原时间、凝血酶时间、活化的部分凝血活酶时间、国际标准化比值显著高于对照组,组间比较差异有统计学意义(P<0.01,P<0.05)。结论:植物血凝素在体内外均能诱发凝集反应,并能激活凝血系统,在临床应用时应注意其对凝血系统的影响。
Objective：To explore the agglutination reaction induced by phytohemagglutinin（PHA） and it＇s effects on coagulation system.Methods：12 Wistar rats were randomly divided into experimental group and control group,which were injected PHA and 0.9%saline into caudal vein respectively.The agglutination of red blood cells in vitro and in vivo,hemolysis,the rats＇ general conditions and coagulation markers of rats were observed.Results：PHA could induce agglutination reaction and hemolysis in vitro and in vivo.The rats in experimental group showed the following performance：piloerection,arched,cured up and trembling;but the rats in control group did not show above performance.The fibrinogen of rats in experimental group was obviously lower than that of rats in control group（P〈0.01）;While the prothrombin time,thrombin time,thromboplastin time and international normalized ratio were obviously higher（P〈0.01,P〈0.05）.Conclusions：PHA can induce agglutination reaction in vivo and in vitro;it also can activate coagulation system.Clinical application should pay attention to its＇ effects on coagulation system.
出处
《承德医学院学报》
2016年第1期12-14,共3页
Journal of Chengde Medical University
关键词
植物血凝素
凝集反应
凝血系统
临床应用
Phytohemagglutinin（PHA）
Agglutination reaction
Coagulation system
Clinical application
