摘要
目的：探讨血凝素诱发的凝集反应对血浆组织因子（TF）、D-二聚体及内皮细胞的影响。方法：42只SPF级SD大鼠（雌雄不限,体重180～200 g）,随机分为生理盐水对照组、不同浓度（5、10和20 g/L）植物血凝素组及灭活的植物血凝素组,每组6只。通过尾静脉向大鼠体内注入生理盐水、植物血凝素及灭活植物血凝素,用ELISA法检测大鼠血浆TF和D-二聚体的水平,透射电镜观察大鼠肺部毛细血管内皮细胞形态。结果：5、10和20g/L植物血凝素组大鼠血浆TF和D-二聚体含量均显著高于生理盐水对照组及相应浓度的灭活植物血凝素组（P〈0.05）。电镜下,与生理盐水对照组相比,各浓度植物血凝素组出现肺毛细血管内皮细胞损伤,表现为内皮细胞肿胀、溶解,胞质疏松,边界不清,线粒体肿胀,基膜增厚。各灭活植物血凝素组内皮细胞结构正常。结论：体内发生的凝集反应可破坏毛细血管内皮细胞,导致凝血纤溶系统紊乱。
AIM： to explore the effects of agglutination on tissue factor（ TF）,D-dimer and endothelial cells in vivo. METHODS： Forty-two SPF SD rats（ male and female,180 ～ 200 g） were randomly divided into normal saline control group,and different concentrations（ 5,10 and 20 g / L） of phytohemagglutinin（ PHA） groups and inactivated PHA groups,with 6 rats in each group. The rats were injected with normal saline,PHA and inactivated PHA through the tail vein respectively. The plasma contents of TF and D-dimer were detected by ELISA. The pulmonary capillary endothelial cells of the rats were observed under electron microscope. RESULTS： The plasma levels of TF and D-dimer were significantly higher in all PHA groups than those in normal saline group and 3 corresponding inactivated PHA groups. Compared with normal saline group,the pulmonary capillary endothelial cells of the rats in each PHA group were damaged,characterized by endothelial cell swelling and dissolution,cytoplasm osteoporosis,unclear boundary,mitochondrial swelling and thick basement membrane. In all inactivated PHA-groups,the structures of the endothelial cells were normal. CONCLUSION： PHA-induced agglutination damages capillary endothelial cells and causes the disorders of the coagulation-fibrinolysis system.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2016年第11期2088-2090,2112,共4页
Chinese Journal of Pathophysiology
基金
河北省重大实验课题(No.ZD2013047)
