摘要
以野生长爪沙鼠为对象,通过外源注射植物血凝素(Phytohemagglutinin,PHA)和磷酸盐缓冲液,分别测定了夏季和冬季沙鼠注射前、注射24h和48h后体重和静止代谢率(Resting metabolic rate,RMR)的变化,以及48h后沙鼠足增重量和白细胞总数(White blood cell counts,WBCs)的变化,以检测沙鼠的免疫功能和能量代价的性别和季节差异。结果显示:1)注射PHA能显著增加沙鼠足重和白细胞的总数,夏季白细胞的总数高于冬季;2)冬季沙鼠体重高于夏季,雄鼠大于雌鼠,但PHA处理对注射前后沙鼠的体重无影响;3)冬季RMR高于夏季,雄鼠大于雌鼠,但PHA处理对沙鼠的RMR无影响,PHA处理对注射前后沙鼠的RMR也无影响。这些结果表明,长爪沙鼠对PHA的反应具有季节差异,但无性别差异,也没有发现明显的能量学代价,这可能与野外环境条件的大幅度波动(如环境温度和食物条件等)和沙鼠的繁殖状态有关。
To evaluate the season and gender differences,as well as the cost of mounting phytohemagglutinin（PHA） challenged immune response,we measured the variations of body mass and resting metabolic rate（RMR） before and after 24 h and 48 h by injections of PHA and PBS in male and female Mongolian gerbils in different seasons,as well as the changes of increased foot mass and the total number of white blood cell counts（WBCs） at the end of the experiment.The results showed that 1） Increased foot mass（%） was significantly increased in PHA-challenged group than control gerbils in either gender in either season.The total number of WBCs was higher in summer than winter.2） Body mass was higher in winter than summer,and males were higher than females,but no different at different time points within PHA-challenged and control gerbils.3） RMR was higher in winter than summer,and males were higher than females,but no different at different time points within PHA-challenged and control gerbils.We conclude that the PHA responses show significant seasonal differences in wild Mongolian gerbils,but fail to find marked sexual differences and energetic costs,suggesting that such short enhancement of immune functions may be related with wide fluctuations of environmental conditions（ambient temperature and food availability） and animal＇s reproductive cycle.
出处
《兽类学报》
CAS
CSCD
北大核心
2011年第3期284-290,共7页
Acta Theriologica Sinica
基金
农业虫害鼠害综合治理研究国家重点实验室开放研究基金资助项目(IPM1001)
中国科学院重要方向资助项目(KSCX2-YW-Z-1021)
关键词
长爪沙鼠
植物血凝素
静止代谢率
性别
季节变化
Gender
Mongolian gerbils
Phytohemagglutinin
Resting metabolic rate
Season
