摘要
以戈斯纳(Gosner,1960)分期表为标准,测定了28、38、42、44、45和46期野生花背蟾蜍(Pseudepidalea raddei)身体和消化器官的大小,并通过断尾或心脏穿刺采血技术,测定了各型白细胞的百分比及嗜中性粒细胞和淋巴细胞的比值(N/L的比值)的变化。结果表明:体重、体全长及尾长随发育阶段先升后降,而重长比和体长则一直增加;小肠长度和湿重均在38期最高,后者显著高于42期;肝湿重从28期至45期逐渐增加,但46期时下降;嗜中性粒细胞的百分比45期显著高于38期,嗜酸性粒细胞的百分比44期显著高于42和45期;淋巴细胞、嗜碱性粒细胞和单核细胞的百分比及N/L的比值均不随发育阶段而变化。这些结果提示,花背蟾蜍蝌蚪能量获取器官和能量贮存器官的大小分别在变态高峰期和登陆前达到最大值,嗜中性粒细胞和嗜酸性粒细胞的比例变化有助于蝌蚪从水生过渡到陆地生活、完成变态发育过程。
The developments of body size and digestive organ size in wild tadpoles of Mongolian toads （Pseudepidalea raddei） during the Gosner stages 28, 38, 42, 44, 45, and 46 （G28, G38, G42, G44, G45, and G46） were recorded following Gosner staging table （Gosner, 1960）. Blood samples were collected by cutting tail or cardiac puncture. The percentages of different subtypes of leukocyte and the ratio of neutrophils to lymphocytes （ratio of N/L） were measured. The results showed that body mass, total body length, and tail length initially increased, and then gradually decreased over the developmental stages. Body length and the ratio of body mass to body length were increased over all the developmental stages. The length and wet mass of small intestine reached the peak in G38, with the wet mass being higher than that in G42. Liver wet mass increased from G28 to G45 tadpoles, but decreased in G46. Percentage of neutrophils in G45 was greater than in G38. Percentage of eosinophils was greater in G44 than that in G42 and G45. Percentages of lymphocytes, basophils and monocytes, as well as the ratio of N/L did not change across different developmental stages. These results indicate that the sizes of energy acquisition and energystorage organs reach the maximum value at metamorphic peak and before landing, respectively. The changes of the proportions of neutrophils and eosinophils are beneficial to the transition from aquatic to terrestrial environment, as well as to the completion of metamorphosis development.
作者
连丽燕
高慧清
孙嘉珞
孙思琪
张琼文
张志强
LIAN Li-yan, GAO Hui-qing, SUN Jia-luo, SUN Si-qi. ZHANG Qiong-wen, ZHANG Zhi- qiang(College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China)
出处
《生态学杂志》
CAS
CSCD
北大核心
2018年第4期1204-1210,共7页
Chinese Journal of Ecology
基金
国家自然科学基金项目(31170379)
2016年安徽农业大学大学生科技创新基金项目资助
关键词
花背蟾蜍
蝌蚪
淋巴细胞
嗜中性粒细胞
嗜酸性粒细胞
消化器官大小
Mongolian toad （Pseudepidalea raddei） ~ tadpole
lymphocyte
neutrophil
eosino-phil
digestive organ size.
