摘要
目的:研究补阳还五汤(益气活血)、血府逐瘀汤(活血)、四君子汤(益气)等3种不同治法的中药复方含药血清对脂多糖诱导的血管内皮细胞Toll样受体4及下游信号转导通路主要元件的影响,探讨不同治法的中药复方防治动脉粥样硬化的机制。方法:新西兰大耳白兔随机分为正常组、补阳还五汤组、血府逐瘀汤组、四君子汤组,每组5只。正常组以20 m L生理盐水ig;3个中药复方组分别以20 m L补阳还五汤(6.7 g·kg-1),血府逐瘀汤(3.6 g·kg-1),四君子汤(1.6 g·kg-1)ig,连续ig 7 d。在末次ig给药2 h后,心脏取血,离心后制备中药含药血清。血管内皮细胞随机分为空白组,模型组,阿托伐他汀组(10μmol·L-1),补阳还五汤组,血府逐瘀汤组,四君子汤组6组,用脂多糖(1 mg·L-1)刺激2 h后分别加入含10%阿托伐他汀药、中药复方含药血清干预,24 h后收集细胞,用荧光定量PCR方法测定Toll样受体4(TLR4),下游髓样分化因子88(My D88),肿瘤坏死因子受体相关因子6(TRAF-6)及核因子κB(NF-κB)的基因表达,用Western blot方法测定TLR4和NF-κB蛋白表达。结果:模型组用脂多糖刺激内皮细胞后引起TLR4,My D88,TRAF-6及NF-κB的表达升高,与空白组比较,差异有统计学意义(P<0.01)。用补阳还五汤和血府逐瘀汤含药血清干预之后显著抑制各项指标的表达,与模型组比较,差异有统计学意义(P<0.05),而四君子汤组各项指标无明显变化,与模型组比较,差异无统计学意义。结论:补阳还五汤和血府逐瘀汤可抑制TLR4及下游信号转导通路主要元件的表达,这可能是2种方剂防治动脉粥样硬化作用的机制之一。
Objective： To investigate the effect of drug serum by Chinese herbal compound prescription in Buyang Huanwu Tang（ BHT）,Xuefu Zhuyu Tang（ XZT） and Sijunzi Tang（ ST） on the expression of Tolllike receptor-4（ TLR4） and main components of downstream signal transduction pathways,and to study their possible anti-atherosclerotic mechanism. Method： New Zealand rabbits were divided into 4 groups in random：normal group,BHT group,XZT group,and ST group,with 5 rabbits in each group. Rabbits in normal group were given gastric perfusion of 20 m L normal saline,rabbits in three Chinese herbal compound groups respectively received gastric perfusion of 20 m L BHT（ 6. 7 g·kg^-1）,XZT（ 3. 6 g·kg^-1） and ST（ 1. 6 g·kg^-1）,for 7continuous days. Blood was drawn from rabbit＇ s heart 2 h after the last gastric perfusion,and then the drug serum was prepared after centrifugation. Vascular endothelial cells were randomly divided into 6 groups： blank group,model group,Atorvastatin group（ 10 μmol·L- 1）,BHT group,XZT group and ST group. After stimulation with LPS（ 1 mg·L- 1） for 2 h,drug serum containing 10% control western medicine and drug serum containing Chinese herbal compound were added separately for intervention. Cells were collected after 24 hours. The gene expression of TLR4,myeloid differentiation factor 88（ My D88）,tumor necrosis factor-associated factor 6（ TRAF-6） and nuclear factor-κB（ NF-κB） were measured with RT-q PCR. Western blotting method was used to analyze the expression of TLR4 and NF-κB protein. Result： expressions of TLR4,My D88,TRAF-6 and NF-κB in model group were increased significantly after LPS stimulation,with statistical difference compared with blank group（ P〈0. 01）. Intervention by BHT and XZD could significantly decrease the expression of TLR4,My D88,TRAF-6 and NF-κB,with statistical difference compared with model group（ P〈0. 05）. However,ST group had no significant changes in various indicators,with no statistical difference co
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2016年第1期121-124,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81160476)
