摘要
目的探讨补阳还五汤对同型半胱氨酸(homocysteine,Hcy)致动脉粥样硬化(AS)的保护作用与核因子(NF)-κB的关系。方法给予ApoE-/-小鼠Hcy(0.1 g.kg-1)灌胃8周后造成动脉粥样硬化(AS)模型,治疗组给予补阳还五汤(5,10,20g.kg-1)进行灌胃。8周后HE染色观察和测量主动脉根部AS斑块面积、血管壁面积,检测主动脉NF-κB活性、活性氧(ROS)含量、I-κBα蛋白表达变化。结果模型组AS病变明显,与正常对照组相比,主动脉组织中ROS含量显著升高(P<0.01),I-κBα蛋白表达明显降低,NF-κB活性显著激活;不同剂量的补阳还五汤改善AS病变的同时显著降低血管组织中ROS含量、促进I-κBα蛋白表达升高,抑制NF-κB的活化。结论补阳还五汤可能通过抑制NF-κB的活化达到抗Hcy所致动脉硬化的作用。
OBJECTIVE To investigate the effects of Buyanghuanwu decoction on preventing atherosclerosis induced by Hcy in rats in vivo. METHODS The model of atherosclerosis in rats was induced by intragastric garage of Hcy. The intervention group was admin- stered Buyanghuanwu decoction（5~10, 20 g·kg^-1 ·d-1 ）： After 8 weeks treatment, the area Of atherosclerotic plaques, blood vessel walls and their ratio, and ROS contention in the aorta were analyzed. The positive cell ratio of NF-κB P65 in vascular was detected by im- munohistochemistry, and the expression of I-KBct was determined by Western-bltting. RESULTS The rats in the model group showed evident AS lesions which had significant difference with Others groups. Compared with control group, the ROS content of model group was elevated （P 〈 0. 01 ）, the I-κBαprotein in the vaScular tissue was significantly reduced, and NF-κB was significantly activated. Different doses of Buyanghuanwutang decoction could decrease ROS content （P 〈 0. 01 ）, improve I-κBα protein and inhibit the activation of NF- κB. CONCLUSION Buyanghuanwutang decoction＇can prevent atherosclerosis induced by Hcy via inhibiting NF-κB-dependent pathway.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2012年第2期104-108,共5页
Chinese Pharmaceutical Journal
基金
国家自然基金项目资助课题(30960505)
国家973项目资助课题(2010CB530603)
