摘要
建立多波长HPLC法同时测定过敏性鼻炎颗粒中绿原酸、甘草苷、迷迭香酸、牛蒡苷、甘草酸、五味子醇甲、胡薄荷酮的方法。色谱柱为Waters Symmetry C18(4.6 mm×250 mm,5μm);以乙腈-0.1%磷酸水溶液为流动相,梯度洗脱,绿原酸、迷迭香酸检测波长为327 nm,甘草苷、牛蒡苷检测波长为280 nm,甘草酸、五味子醇甲、胡薄荷酮检测波长为250 nm;柱温25℃,进样量10μL。绿原酸在1.19-59.50μg?m L^(-1)范围内呈良好线性关系(r=0.999 8),平均回收率为100.95%;甘草苷在1.51^(-1)50.70μg?m L^(-1)范围内呈良好线性关系(r=0.999 1),平均回收率为100.38%;迷迭香酸在3.40-68.08μg?m L^(-1)范围内呈良好线性关系(r=0.999 9),平均回收率为101.02%;牛蒡苷在56.15^(-1)123.00μg?m L^(-1)范围内呈良好线性关系(r=0.999 9),平均回收率为100.39%;甘草酸在21.54-430.80μg?m L^(-1)范围内呈良好线性关系(r=0.999 8),平均回收率为97.09%;五味子醇甲在2.57-51.34μg?m L^(-1)范围内呈良好线性关系(r=0.999 9),平均回收率为99.19%;胡薄荷酮在0.50^(-1)0.00μg?m L^(-1)范围内呈良好线性关系(r=0.999 9),平均回收率为100.35%。所建方法简便可靠,重复性好,可作为过敏性鼻炎颗粒中有效成分的测定方法。
This study was aimed to establish a simultaneous determination method of chlorogenic acid, liquiritin, rosmarinic acid, arctiin, glycyrrhizic acid, schisandrin, menthone in Guo-Ming-Xing Bi-Yan（GMXBY） granules by HPLC under multiple UV wavelengths. Waters Symmetry C18 column（4.6 mm × 250 mm, 5 μm） was used as the chromatographic column. Acetonitrile-0.1% phosphoric acid water solution was used as the mobile phase with gradient elution. The detection wavelength of chlorogenic acid and rosmarinic acid was 327 nm; that of liquiritin and arctiin was 280 nm; that of glycyrrhizic acid, schisandrin and menthone was 250 nm. The column temperature was 25℃. The injection volume was 10 μL. Chlorogenic acid showed a good linear relationship in the range of 1.19-59.50 μg？m L^-1（r = 0.999 8）. The average recovery rate was 100.95%. Liquiritin showed a good linear relationship in the range of 1.51^-150.70 μg？m L^-1（r = 0.999 1）. The average recovery rate was 100.38%. Rosmarinic acid showed a good linear relationship in the range of 3.40-68.08 μg？m L^-1（r = 0.999 9）. The average recovery rate was 101.02%. Arctiin showed a good linear relationship in the range of 56.15^-1 123.00 μg？m L^-1（r = 0.999 9）. The average recovery rate was 100.39%. Glycyrrhizic acid showed a good linear relationship in the range of 21.54-430.80 μg？m L^-1（r = 0.999 8）. The average recovery rate was 97.09%. Schisandrin showed a good linear relationship in the range of 2.57-51.34 μg？m L^-1（r = 0.999 9）. The average recovery rate was 99.19%. Menthone showed a good linear relationship in the range of 0.50^-10.00 μg？m L^-1（r = 0.999 9）. The average recovery rate was 100.35%. This established method was simple and reliable with good reproducibility, which can be used as the determination method of active components in GMXBY granules
出处
《世界科学技术-中医药现代化》
2015年第11期2252-2259,共8页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
科学技术部国家重大新药创制项目(2013ZX09402203):现代中药创新集群与数字制药技术平台
负责人:王振中
