摘要
目的：建立高效液相色谱（HPLC）法测定地桃花中银锻苷的含量。方法：采用Agilent Eclipse XDB C18色谱柱（250 mm×4.6 mm,5μm）;流动相为乙腈-水,梯度洗脱;流速为1.0 m L·min-1;柱温为25℃;检测波长为320 nm。结果：银锻苷在进样量为0.02~1.00μg范围内,进样量与峰面积线性关系良好（r=0.999 5）;最低检出限为0.74 ng;平均回收率为98.87%（相对标准偏差RSD为1.48%,n=6）。结论：本方法简便准确、快速可靠,对地桃花药材的质量控制具有参考价值。
Objective： To establish an HPLC method for quantification of tiliroside in Urena lobata L.Methods： The separation was performed on an Agilent Eclipse XDB C18column（ 250 mm × 4. 6 mm,5 μm） with a gradient elution of acetonitrile and water as mobile phase. The column temperature was 25 ℃ and the detection wavelength was set at 320 nm. Results： A good linear correlation was observed within the range of 0. 02 ~ 1. 00 μg for tiliroside（ r = 0. 999 5）,the minimum detection limit was 0. 74 ng,and the average recovery was 98. 87%（ RSD = 1. 48%,n = 6）. Conclusion： The method is simple,accurate and rapid. It could be used for the quality control of Urena lobata L.
出处
《中国新药杂志》
CAS
CSCD
北大核心
2015年第24期2865-2867,共3页
Chinese Journal of New Drugs
基金
国家药典委员会2015年版<中华人民共和国药典>资助项目
国家工信部中药材扶持资助项目
