摘要
目的：建立同时测定血清中两种探针药物（咖啡因、氯唑沙宗）浓度的高效液相色谱法。方法：采用迪马C18色谱柱（250mm×4．6mm，5μm）；流动相：乙腈-0．02mol·L^-1磷酸二氢钾溶液（1：3，v／v），含0．01mol·L^-1三乙胺；流速：1．5mL·min^-1。；紫外检测波长：280nm；柱温：30℃。结果：咖啡因在2．5～12．5μg·mL^-1。、氯唑沙宗在2．5～12．5μg·mL^-1的浓度范围内线性关系良好，最低检测限分别为0．1μg·mL^-1。、0．4μg·mL^-1。，回收率分别为103．70％±4．36％、102．82％±4．39％。结论：本法操作简便，灵敏度高，快速可靠，可用于血清中咖啡因和氯唑沙宗的浓度测定。
Objective：To establish an RP-HPLC method for the determination of probe drugs（caffeine and chlorzoxazone） in serum. Methods：Diamonsil C 18 （250× 4.6 mm, 5 μm） column was used. The mobile phase consisted of acetonitrile-0.02 mol·L^-1 buffer phosphate （25： 75, pH 6,28） and 0.01 mol· L^- 1 triethylamine, the flow rate was 1.5 mL ·min^-1. The detection wavelength was 280 nm. The column was kept at 30℃. Results Linear ranges of caffeine and chlorzoxazone in serum were 2.5 - 12.5 μg·mL^-1 and 2. 5 - 12.5 μg·mL^-1, respectively. The limit detection were 0.1 μg·mL^-1,0.4 μg· mL^-1 ; and average recoveries of method were tively. Conclusion ： The method is simple, rapid, and caffeine and chlorzoxazone in serum. 103.70% ±4.36%, 102.82% ±4.39%, respecreliable, and it is suitable for the determination of
出处
《药学与临床研究》
2008年第3期235-237,共3页
Pharmaceutical and Clinical Research
