摘要
为了解河南省市售小麦粉的安全状况,收集整理河南省市售小麦粉。采用Filmplate^(TM)测试片法测定样品的菌落总数、霉菌和酵母菌计数及大肠菌群,采用酶联免疫法(Enzyme–Linked Immuno Sorbent Assay,ELISA)测定样品中呕吐毒素(DON)、黄曲霉毒素B_1(AFB_1)及赭曲霉毒素A(OTA)三种常见真菌毒素的含量。结果显示:小麦粉样品受微生物污染面积较广,但程度可控。菌落总数、霉菌酵母菌数、大肠菌群数主要集中范围分别是10~2~10~4、10~2~10~3、10~2~10~3 CFU/g;DON、AFB_1及OTA含量超限率分别为0.00%、13.85%、7.69%,检出率依次为69.23%、52.31%及84.62%,污染程度不同,污染范围均较为广泛。
In order to know the security situation ofcommercial wheat flour inHenanProvince, commercial wheat flour ofHenan werecollected and arranged.The total numbers ofcolonies,moulds and yeastscoliforms weremeasured by FilmplateTM test.Threecommonmycotoxinscontents, including deoxynivalenol（DON）,aflatoxin B1（AFB1） and ochratoxinA（OTA）,weremeasured by Enzyme–Linked ImmunoSorbentAssay（ELISA）.The results showed that wheat flour samples were polluted bymicrobialcontamination widely,but the degree was incontrol.The total numbers ofcolonies,moulds and yeasts,andcoliforms seperately focused on 102-104,102-103,102-103CFU/g;The over–limit rates of DON,AFB1 andOTA were 0.00%,13.85%,7.69%,respectively. The detection rates were 69.23%,52.31%,84.62%.The degrees ofcontamination were different,while the areas were all extensive.
出处
《粮食与油脂》
北大核心
2015年第12期64-66,共3页
Cereals & Oils
基金
河南省小麦产业技术体系建设专项(S2010–01–G06)
国家小麦产业技术体系专项(CARS–03)
关键词
市售小麦粉
微生物
真菌毒素
污染
commercial wheat flour
microorganism
mycotoxins
contamination
