摘要
目的探讨溶酶体相关4次跨膜蛋白质B（LAPTM4B）基因在良恶性前列腺组织中的表达差异及异常表达的LAPTM4B与前列腺癌的关系。方法采用已知微阵列（Microarray）数据分析LAPTM4B在良恶性前列腺组织中的表达差异。利用免疫组织化学技术对100例前列腺癌和80例良性前列腺标本中LAPTM4B的蛋白表达水平进行分析。结果LAPTM4B的DNA水平在良恶性前列腺组织中差异有统计学意义（P〈0．01）；转移性前列腺中LAPTM4B的DNA水平明显高于原发前列腺癌（P〈0．01）。LAPTM4B的mRNA水平在高Gleason评分患者中明显增高（P〈0．05）。LAPTM4B的蛋白表达在良恶性前列腺组织中差异有统计学意义（P〈0．01）。在不同水平前列腺特异抗原（PSA）前列腺癌患者中LAPTM4B蛋白表达差异有统计学意义（P〈0．01）；在不同Gleason评分的前列腺癌患者间LAPTM4B蛋白表达差异有统计学意义（P〈0．01）；LAPTM4B蛋白表达在PSA生化复发的前列腺癌患者中明显增高（P〈0．05）。结论在前列腺癌组织中LAPTM4B基因的表达异常，上调的LAPTM4B与前列腺癌具有相关性，可能为潜在的前列腺癌标志物。
Objective To analyze the expression of lysosome - associated protein transmembrane 4 beta （LAPTM4B） in prostate tissues and investigate the relationship with clinicopathological variables in prostate cancer. Methods Use available microarray datasets to test the expression of LAPTM4B in pros- tate tissues. In 100 prostate cancer （PCa） and 80 benign prostatic hyperplasia （BPH） samples, we per- formed immunohistochemical staining to validate expression of LAPTM4B. Results Microarray data indi- cated that that LAPTM4B DNA copy number was significantly increased in PCa tissues relative to normal prostate tissues （P 〈 0. 01 ）. Similarly, increased LAPTM4B DNA copy number was found in metastatic PCa （P 〈 0. 01 ）. mRNA level of LAPTM4B is significantly over- expressed in patients with higher Glea- son score （ P 〈 0. 05 ）. LAPTM4B protein was overexpressed in PCa tissues compared with the BPH （ P 〈 0. 01 ）. LAPTM4B protein expression was significantly upregulated in PCa patients with, higher preopera- tive prostate specific antigen （PSA, P 〈 0.01 ） ; in patients of higher Gleason score LAPTM4B protein was significantly increased （P 〈 0. 01 ） , and in patients with BCR LAPTM4B protein expression was significant- ly higher （P 〈 0.05 ）. Conclusion Overexpressed LAPTM4B is associated with some PCa clinicopatho- logical variables and is a potential PCa molecular marker.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2015年第12期2964-2966,共3页
Chinese Journal of Experimental Surgery
关键词
溶酶体相关4次跨膜蛋白质β
前列腺癌
免疫组织化学
Lysosome - associated protein transmembrane 4
Prostate cancer
Immunohistochemical staining
