摘要
目的:探讨PPARγ上调对PCOS患者卵巢颗粒细胞P450arom mRNA表达的调控作用及对T转化效应的影响。方法:提取10例雄激素正常的PCOS患者(NA-PCOS组)、10例伴高雄激素血症的PCOS患者(HA-PCOS组)及10例仅因输卵管因素不孕患者(对照组)的卵巢颗粒细胞,分别给予0、1、10 nmol/L的PPARγ激动剂罗格列酮培养48 h后,采用RT-PCR测定PPARγ、P450arom mRNA的表达,采用ELISA法测定培养液中E2值,计算T转化率。结果:PPARγ上调后,3组患者P450arom mRNA的表达均降低,T转化率下降,尤其是HA-PCOS组(P<0.05)。PPARγ上调(10 nmol/L罗格列酮处理)后,HA-PCOS组卵巢颗粒细胞中PPARγmRNA的表达水平和T转化率负相关,P450arom mRNA的表达水平和T转化率正相关(r=-0.633、0.829,P<0.05)。结论:PPARγ可能通过抑制P450arom参与PCOS高雄激素血症的发生。
Aim：To explore the effects of up-regulation of PPARγon expression of P450arom mRNA and T transfor-ming effects in the ovarian granulosa cells from patients with polycystic ovarian syndrome （ PCOS） .Methods：A total of 30 patients were included, with 10 in NA-PCOS group（PCOS with normal androgen）,10 in HA-PCOS group（PCOS with hy-perandrogenism） and 10 in control group.Granulosa cells were prepared and treated with 0,1,10 nmol/L rosiglitazone for 48 h.The expressions of PPARγmRNA and P450arom mRNA were measured by RT-PCR.After intervention, the E2 value in broth was determined by ELISA and T transforming rate was calculated .Results：After the up-regulation of PPARγ, the expression of P450arom mRNA and the T transforming rate in the 3 groups were decreased,especially in the HA-PCOS group.In HA-PCOS group（being treated by 10 nmol/L rosiglitazone）,the expression of PPARγmRNA was negatively as-sociated with T transforming rate,and the expression of P450arom mRNA was positively associated with T transforming rate （r=-0.633,0.829,P〈0.05）.Conclusion： PPARγmay participate in the pathogenesis of PCOS with high androgen level by down-regulating the expression of P450arom.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2015年第6期790-793,共4页
Journal of Zhengzhou University(Medical Sciences)
基金
河南省科技攻关省部共建项目201201001
