摘要
目的:建立舒心安神胶囊中栀子苷的含量测定方法。方法:采用高效液相法,以Diamonsil C18柱(4.6 mm×150 mm)为色谱柱,以甲醇-水(19∶81)为流动相,流速1 m L/min,柱温30℃,2998紫外检测器,检测波长238 nm。结果:胶囊中栀子苷与样品中其他组分能基线分离,阴性对照无干扰;栀子苷在67 ng^1 340 ng范围内呈良好线性关系,r=0.999 9;精密度实验RSD=0.84%(n=6);样品在10 h内稳定;重复性试验的RSD为1.82%;平均回收率为99.42%,RSD=0.88%(n=6)。结论:该方法简便快捷,结果可靠,可以作为舒心安神胶囊的质量控制方法。
Objective：To establish a method of content determination of gardenoside in Shuxin Anshen capsule. Methods：HPLC was performed on Diamonsil Column C18 （4.6 mm×150 mm）,methanol-water （19∶81）served as mobile phase,the flow rate was 1 mL/min,column temperature was 30 ℃,2998 UV detector was used as detector,the wavelength was 238 nm. Results：Gardenoside and other components from the capsule would be separated on baseline,negative control sample would not influence on sample;the liner range was 67 ng^1 340 ng for gardenoside;the experimental of precision test in RSD was 0.84%（n=6）;the sample kept stability within 10 hours;repeatability test of RSD was 1.83%;the average recov-ery was 99.42% with corresponding RSD was 0.88%（n=6）. Conclusion：The method is not only simple and sensitive,but also reproducible and accurate,which can be uesd for the quality control of Shuxin Anshen Capsule.
出处
《山西中医学院学报》
2015年第4期34-35,共2页
Journal of Shanxi College of Traditional Chinese Medicine
关键词
栀子苷
HPLC
含量测定
Gardenoside
HPLC
content determination
