摘要
目的:建立玉簪清咽十五味丸中栀子苷的含量测定方法。方法:十八烷基硅烷键合硅胶为填充剂(Diamonsil C185μm 4.6mm×250mm);流动相:乙腈-水(15∶85);检测波长:238nm;流速:1.0ml/min;柱温:30℃;理论板数按栀子苷峰计算应不低于1500。结果:栀子苷线性范围在0.128～0.765μg,r=0.9998,平均回收率99.33%,RSD=1.20%(n=6)。结论:该方法简便、快捷、准确、重复性好,可用于该蒙药的质量控制。
Objective： To establish the method to determine the concentration of gardenoside in Yuzhan Qingyan fifteen flavor pills. Methods： HPLC conditions were as follows： VP-ODS column （Diamonsil C18 5μm 4.6mm×250mmm）, a mobile phase of CH3CN-H20 （15： 85）, detection wavelength 238 nm, flow velocity 1.0ml/min, column temperature 30℃, theoretcal plate number not lower than 1500 at the peak. Results： Gardenoside was linear within the range of 0.128-0.765μg with a correlation coefficient of 0.9998. The average recovery percentage was 99.33% and RSD was 1.20% （n=6）. Conclusion： The method is evidenced to be simple, timesaving and accurate with a favorable reliability; therefore it can be used effectively for the quality control ofYuzhan Qingyan fifteen flavor pills.
出处
《中医临床研究》
2012年第3期16-19,共4页
Clinical Journal Of Chinese Medicine
基金
国家973计划前期研究专项课题
题目:特色蒙药材及复方制剂质量标准与主要药效研究
课题编号:2011CB512011
