摘要
目的探讨T-cadherin蛋白与基因在混合细胞型霍奇金淋巴瘤患者中的表达情况及其临床意义。方法分别采用免疫组化法和RT-PCR同时检测45例混合细胞型霍奇金淋巴瘤和20例淋巴结反应性增生淋巴结组织及其骨髓mRNA中Tcadherin的表达情况,分析其与患者临床病理特征和预后的关系。结果 T-cadherin蛋白在混合细胞型霍奇金淋巴瘤阳性表达率31.1%(14/45)显著低于淋巴结反应性增生90.0%(18/20);T-cadherin mRNA的相对表达量在混合细胞型霍奇金淋巴瘤患者显著少于淋巴结反应性增生患者(P<0.05)。混合细胞型霍奇金淋巴瘤中T-cadherin表达与患者TNM分期和结外累及有关(P<0.05),与患者年龄、性别、WBC、PLT、Hb、LDH和IPS评分无相关(P>0.05)。多因素Logistic回归分析显示,TNM分期和Tcadherin阴性表达是混合细胞型霍奇金淋巴瘤患者结外累及的独立危险因素。经过Log-rank检验显示,混合细胞型霍奇金淋巴瘤患者T-cadherin阴性表达和T-cadherin阳性表达的2年生存率分别是83.9%和92.9%(P>0.05);3年生存率分别是71.0%和85.7%(P>0.05);5年生存率分别是41.9%和78.6%(P<0.05),Kaplan-Meier生存分析显示T-cadherin阴性表达5年生存率明显低于T-cadherin阳性表达组(P<0.05)。结论 T-cadherin表达减少和混合细胞型霍奇金淋巴瘤患者TNM分期和结外累及有相关性,T-cadherin可能成为评价混合细胞型霍奇金淋巴瘤预后相关的一种新型分子标记物。
Objective To investigate the expression level and clinical significance of T-cadherin protein and gene in the mixed cell type of hybrid hodgkin lymphoma.Methods The lymph nodetissue samples were obtained from 45 hybrid Hodgkin lymphoma patients and 20 reactive hyperplasia lymphoid patients.The expression level of T-cadherin of these samples were detected by immunohistochemical staining and RT-PCR.The relationship of clinic pathological features and prognosis in hybrid Hodgkin lymphoma patients were analyzed by the expression level of T-cadherin.Results The positive expression rates of T-cadherin in protein hybrid hodgkin lymphoma and reactive hyperplasia lymphoid tissue specimens were 31.1%（14/45）and 90.0%（18/20）（P〈0.05）.T-cadherin mRNA relative expression in hybrid Hodgkin lymphoma was significantly lower than reactive hyperplasia lymphoid tissuespecimens（P〈0.05）.The expression of T-cadherin was significantly correlated with TNM and lymph node involvement（all P〈0.05）,but not significantly correlated with age,gender,WBC,PLT and Hb count,LDH and IPS grade（all P〈0.05）.Multivariate logistic regression analysis demonstrated TNM stage and T-cadherin negative expression were independent factors of lymph node involvement.Log-rank test revealed the two-year survival rate in hybrid Hodgkin lymphoma patients of T-cadherin negative and positive expressionwere 83.9% and 92.9%（P〉0.05）,three-year survival rate were 71.0% and 85.7%（P〉0.05）,five-year survival rate were 41.9% and 78.6%（P〈0.05）.Kaplan-Meier survival analysis showed the five-year survival in T-cadherin negative expressionwas lowersignificantly than T-cadherin positive expression（P〈0.05）.Conclusion T-cadherin was low expression in hybrid Hodgkin lymphoma and correlated with TNM and lymph node involvement.T-cadherin may be a new molecular marker to evaluate the prognosis of hybrid hodgkin lymphoma.
出处
《重庆医学》
CAS
北大核心
2015年第33期4647-4650,共4页
Chongqing medicine
基金
贵州省科技厅计划项目(黔科合J字[2009]2175号)
