摘要
为探究棚室连作甜瓜有效的生物防治方法,研究生防放线菌Act12对棚室连作甜瓜根域微生物区系变化的影响。结果表明:Act12菌株在整个甜瓜生育期具有较好的定殖稳定性,且在第6周存活数量达到最高,根区及根表活菌数分别为4.26×106 cfu·g-1和7.84×106 cfu·g-1;Act12同时促进了根域细菌和放线菌数量的增长以及真菌数量的减少,当接种量为5.0g·kg-1时,在根区及根表细菌/真菌分别较CK增加了295.53%和253.36%,放线菌/真菌增加了479.10%和297.63%,说明Act12生防菌促进了土壤中细菌的增加及真菌的减少;Act12同时显著降低镰刀菌在根域真菌中的比例,当接种量为5.0g·kg-1时,根区及根表镰刀菌比例分别较对照降低了5.05和9.30百分点。
The the effect of inoculating with biocontrol actinomyces Act12 on microbial flora of melon rooting zone was studied, for biocontrol actinomyces greenhouse muskmelon repair and lay the theoretical foundation of continuous cropping obstacle. The results indicated that Actl2 had an expectant colonization stability, the num- ber of biocontrol strains reached a high level after inoculated 6 weeks,4.26× 108 cfu.g^-1 survived in the rhizo- sphere soil and 7.84 × 10^6 cfu·g^-1 survived in the root surface. Actl2 could increase the quantity of bacteria and actinomycetes, reduce the fungi number in melon rooting zone. After inoculation with 5.0 g· kg^-1 of strains, the B/F in the melon rhizosphere soil and root surface soil were increased by 295.53% and 253.36% ,the A/F in- creased by 479.10% and 297.63% than contral,Actl2 could help the microbial flora of melon rooting zone soil changed markedly from fungi type to bacteria type. In addition, the fusarium percent of fungi were decreased significantly,the radio of fusarium in the melon rhizosphere soil and root surface soil inoculated by 5.0 g. kg-1 preparetion decreased markedly by 5.05 and 9.30 percent point respectively.
出处
《黑龙江农业科学》
2015年第11期55-59,共5页
Heilongjiang Agricultural Sciences
基金
黑龙江省农业科学院创新基金重点资助项目(ZD005)
关键词
生物防治
甜瓜枯萎病
放线菌
微生态
biological control
melon wilt
actinomyces
micro-ecosystem
