摘要
目的通过小鼠子宫内膜异位症模型的建立,观察不同时期和不同剂量四氯二苯并二恶英(tetrachlorodibenzo-p-dioxin,TCDD)暴露对子宫内膜异位症种植成功率的影响。方法选择60只已孕无特殊病原体(specific pathogen free,SPF)级C57BL/6小鼠,对其雌性子代在其不同的发育阶段给予TCDD处理,分为5组,即0/0、0/3、3/0、3/3、3/10组。其生后第70天采用小鼠自体子宫内膜移植法建立小鼠子宫内膜异位症模型,术后第3、6、9周各给1次TCDD(3μg/kg),术后12周处死小鼠,观察不同种植异位灶(卵巢、腹膜、腹部皮下)的生长情况。结果各组间皮下种植成功率差异无统计学意义(P>0.05),腹膜和卵巢部位种植成功率差异有统计学意义(P<0.05)。腹膜种植成功率在3/3组显著高于0/0组(P<0.05)。0/0组内皮下、腹膜及卵巢异位种植成功率差异无统计学意义(P>0.05)。0/3组、3/0组、3/3组、3/10组内不同部位的异位种植成功率差异有统计学意义(P<0.05),0/3组、3/3组、3/10组卵巢的异位种植成功率显著高于皮下(P<0.05)。3/0组卵巢的异位种植成功率显著高于皮下和卵巢(P<0.05)。随暴露剂量的增加异位灶外观由小囊泡发展为大囊泡,内有大量液体积聚,表面张力较大,腹腔组织粘连严重。结论促进子宫内膜异位症的发生及发展,围产期暴露可增加成年期患子宫内膜异位症的风险。
Objective To observe the effects of tetrachlorodibenzo-p-dioxin（TCDD）on the survivor rate of endometriosis planting in different periods and with different dose,through the establishment of model of endometriosis.Methods Sixty mice which had pregnancy of specific pathogen free（specific pathogen free,SPF）C57BL/6,treated the female offspring with TCDD on different developmental stages,were divided into five groups 0/0,0/3,3/0,3/3,3/10（control group,adolescent＋ adult exposure group,fetal＋ adult exposure group,fetal＋adolescent＋adult common dose exposure group,fetal＋adolescent＋adult high dose exposure group）.And model of endometriosis was made in the seventieth day after birth by using methods of autologous endometrial transplant（ovarian,peritoneal and abdominal subcutaneous）in mouse.The mice were dealt with TCDD（3μg/kg）after surgery respectively on the 3,6,9weeks and put to death after surgery on 12 weeks,to observe the growth of ectopic focus of different plantations.Results The implanted survivor rate of subcutaneous and ovarian in different groups showed no significant difference（P〉0.05）,but the implanted survivor rate of peritoneal and ovarian plantation showed significant difference（P〈0.05）,the implanted survivor rate of 3/3group was significantly higher than that of group 0/0on peritoneal（P〈0.01）.In group of 0/3,3/0,3/3and 3/10 with TCDD exposure,the survivor rate of ovarian was higher than subcutaneous implantation compared with the control group（P〈0.05）.The study also found that the vesicles of ectopic focus was larger with increasing exposure dose,a large quantity of fluid accumulation,the more dose exposure,the more severe abdominal tissue adhesion.Conclusion TCDD promoted the occurrence and development of endometriosis,perinatalstage exposure increased the incidence of endometriosis in adult stage.
出处
《河北医科大学学报》
CAS
2015年第10期1162-1165,共4页
Journal of Hebei Medical University
