摘要
目的 了解杯状病毒在昆明的分子流行病学特征,为预防和控制腹泻提供科学的参考依据.方法 收集云南省第一人民医院和昆明市儿童医院2014年7月至2015年6月5岁以下腹泻患儿的粪便标本321份,采用RT-PCR的方法检测杯状病毒,并选取部分阳性扩增产物经序列测定分析其基因型.结果 321份粪便标本中HuCV的阳性率为10.59％（34/321）,感染无明显的季节性,检出率在患者性别和年龄组间的差异均无统计学意义;34份HuCV阳性标本中以NoV为主,占94.12％ （32/34）,其中绝大多数为GⅡ型（31/32,96.88％）,仅1株为GⅠ型;SaY共2株,占5.88％（2/34）,分别为GⅠ型和GⅡ型.结论 HuCV是昆明地区5岁以下腹泻患儿的重要病原体之一,GⅡ.4/Sydney2012株是流行优势株.
Objective To acknowledge the molecular epidemiology of HuCV among children with acute diarrhea in Kunming,and to provide scientific basis on prevention and control.Methods 321 specimens from children with diarrhea were collected at First people＇ s Hospital of Yunnan province and Children＇ s hospital of Kunming city between July 2014 and June 2015.RT-PCR were used to detect calicivirus.Partial sequence of RNA dependent RNA polymerase （RdRp） of the positive samples were amplified by RT-PCR,and then the PCR production were purified,sequenced and put into phylogenetic analysis.Results Of the 321 specimens,34 positive specimens were identified and the prevalence was 10.59% （34/321）.HuCV Gastroenteritis did not present any seasonal distribution,and the prevalence rates showed no differences between gender and age groups.Typing and phylogenetic analysis revealed that NoV G Ⅱ was the dominant strains.Conclusions HuCV was one of the most major pathogens among children with diarrhea and G Ⅱ.4/Sydney2012 variant was identified as the predominant strain in Kunming.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2015年第5期430-433,共4页
Chinese Journal of Experimental and Clinical Virology
