摘要
目的了解安徽省哨点医院腹泻患儿中病毒性病原体感染状况和分子流行病学特征。方法收集安徽省两哨点医院2011年1月-2012年12月住院腹泻患儿粪便标本549份,采用酶联免疫吸附实验（enzyme linked immunosorbent assay,ELISA）法检测轮状病毒（rotavirus,RV）抗原,采用聚合酶连锁反应（polymerase chain reaction,PCR）或反转录聚合酶连锁反应（reverse transcription-PCR,RT-PCR）检测RV、诺如病毒（norovirus,NoV）、星状病毒（astrovirus,AstV）和肠道腺病毒（adenovirus,AdV）并分型鉴定。结果 549份粪便标本中RV检出率为22.40%（123/549）,NoV为12.02%（66/549）,AstV为1.82%（10/549）,AdV为1.28%（7/549）,混合感染1.15%（4/549）。对RV G/P分型结果显示G9P[8]为主要流行株,NoV以GII-4为主要流行株。结论安徽哨点医院婴幼儿病毒性腹泻的病原以RV为主,秋冬季高发,存在混合感染。
Objective To study the status of viral pathogen infection and molecular epidemiological characters of infants and young children below 5 years of age with diarrhea in surveillance hospitals from Anhui province. Methods A total of 549 stool samples were collected in two sentinel hospitals during January 2011 to December 2012. The antigen of rotavirus （RV） was detected by using enzyme linked immunosorbent assay （ELISA）. The positive samples tested were identified by genotyping and by using reverse transcription-polymerase chain reaction （RT-PCR）. The samples were tested for norovirus （NoV） , astrovirus （AstV） , and enteric adenovirus （AdV） by using RT-PCR or PCR. Results 123 of 549 specimens （22.40%） were positive for RV antigen, 66 cases （ 12. 02% ） were NoV infections, 10 cases （ 1.82% ） were AstV infections, 7 cases （1.28%） were AdV infections, and 4 cases（ 1.15% ） were mixed infection. The study showded that G9P [ 8 ] of RV and GII-4 of NoV were predominant strains in the hospitals. Conclusions Rotavirus was the predominant pathogen for viral diarrhea with mixed infection in surveillance hospitals from Anhui Province during 2011 -2012. Autumn and winter were the high seasons for viral diarrhea.
出处
《中华疾病控制杂志》
CAS
北大核心
2014年第6期508-511,共4页
Chinese Journal of Disease Control & Prevention
基金
国家艾滋病和病毒性肝炎等重大传染病防治项目(2013ZX10004203-002-004)
关键词
腹泻
婴儿
流行病学方法
基因型
Diarrhea, infantile
Epidemiologic methods
Genotype I
