摘要
研究不同的产酶促进剂(渗透压调节剂、表面活性剂、有机酸盐)对重组枯草芽孢杆菌产氨肽酶的影响,并对氨肽酶的提取工艺进行优化。获得产酶促进剂优化组合为:氯化钾0.1 mol/L、吐温802.4μL/m L、L-谷氨酸钠0.6%、酒石酸钾0.7%;优化培养条件为:装液量60 m L/250 m L、初始p H 7.5、接种体积分数5%、转速220 r/min、培养时间36 h。在上述优化条件下,该重组菌株产氨肽酶酶活达232.5 U/m L,是优化前的3.32倍。该重组氨肽酶发酵液经低速离心、双水相萃取、超滤浓缩提取后,回收率达67.23%,纯化倍数为8.29。
This study aimed to investigate the effects of different enzyme accelerants on the production of aminopeptidase by recombinant Bacillus subtilis and develop an efficient extract process of aminopeptidase. The results indicated that the enzyme accelerants（KCl at 0.1 mol/L,Tween-80 at 2.4 μL/m L,L-glutamate at 0.6%,potassium tartrate at 0.7%） significantly increased the production of aminopeptidase. The cultural conditions were of 60 m L/250 m L of liquid,5%inoculation size,at 37 ℃,initial p H 7.5,shake flask rotation 220 r/min,and cultured time 36 h.Under these conditions,the aminopeptidase activity of recombinant Bacillus subtilis could reach232.5 U/m L. Using the efficient extract process of aminopeptidase,the recovery and the purification fold of the L-leucine aminopeptidase could reach 67.23% and 8.29,respectively.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2015年第8期864-872,共9页
Journal of Food Science and Biotechnology
基金
国家863计划项目(2011AA100905)
关键词
氨肽酶
发酵优化
提取工艺
aminopeptidase
optimization of fermentation
extraction process
