摘要
确定了以α-乙酸萘酯为底物植物酯酶活力测定的最佳条件:底物溶液配制浓度为0.015mol·L-1,磷酸缓冲液pH=6.5,40℃恒温水浴定时反应5min,酶抑制剂SDS浓度为3.0%,加入固兰B盐显色,显色30s,加入1∶1的盐酸调溶液pH<5.2,混合均匀,于535nm测定吸光度值,空白为酶液换成蒸馏水,其它不变。
The optimal condition of determining the activity of plant-esterases was studied： the concentration of α-naphthyl acetate which acted as plant- esterases＇ substrates was 0.015mol·L^-1 , and it reacted in pH = 6.5 phosphate buffer solution for 5min in water bath under 40℃. After that,3.0% SDS was added to deactivated the biocatalyst,then diazo fast blue B used for color rending was used for 30s. At last HCI（1：1） was added to adjust the pH below 5.2. The maximum absorption value of the complex compound was at 535nm. And the distilled water was employed instead of α-naphthyl acetate in control groups.
出处
《食品工业科技》
CAS
CSCD
北大核心
2008年第6期145-147,151,共4页
Science and Technology of Food Industry
基金
黑龙江省绿色食品专项课题(GA02B716-06)
关键词
植物酯酶
α-乙酸萘酯
活力测定
条件优化
plant- esterases
α- naphthyl acetate
determining of activities
optimal condition
