摘要
目的探讨血清RASSF1A启动子甲基化可能作为一种新的肝细胞癌（HCC）标志物在无创性诊断中的价值。方法Methy Light法测定190例HCC、114例肝硬化（LC）、120例慢性乙型病毒性肝炎（CHB）患者和160例健康对照者血清RASSF1A甲基化,结果用甲基化比值（PMR）表示,PMR≥4为阳性。结果 HCC、LC、CHB患者血清RASSF1A甲基化阳性率分别为64.2%（122/190）、17.5%（20/114）、5.0%（6/120）,健康体检者未检出,阳性率差异均有统计学意义（P〈0.01）。RASSF1A甲基化阳性时,其从CHB中鉴别HCC的敏感度为64.2%,特异度为89.8%,AUCRASSF1A=0.796（95%CI为0.746~0.842）;RASSF1A甲基化联合AFP（≥20 ng/L）,其敏感度为80.9%,特异度为93.4%,AUCRASSF1A＋AFP=0.876（95%CI为0.836~0.917）。RASSF1A甲基化阳性与HCC组织学分级、TNM分期及远端转移相关（P〈0.05）,与其他临床病理特点无关。结论血清RASSF1A启动子甲基化对HBV相关的HCC可能具有较高的诊断价值,同时可预测HCC临床进展。
Objective To investigate the promoter methylation of RAS association domain family 1A（ RASSF1A） in serum of the patients with hepatocellular carcinoma（ HCC） and its clinical significance. Methods Serum methylation of RASSF1 A in the 190 HCC patients,114 LC patients,120 CHB patients and 160 healthy controls were detected by Methy Light method which was presented as percentage of methylated reference（ PMR）,and when PMR was ≥4,it would be positive. Results The positive rate of serum RASSF1 A methylation in the HCC patients（ 122 /190）,LC patients（ 20 /114）,CHB patients（ 6 /120） and healthy individuals were respectively 64. 2%,17. 5%,5. 0% and 0. 0%,and there were no statistical significance differences on the positive rates（ P〈0. 01）. When RASSF1 A methylation was positive,from CHB in differentiating,HCC sensitivity was64. 2%,specificity was 89. 8%,AUCRASSF1 Awas 0. 796（ 95% CI was among 0. 746- 0. 842）; when RASSF1 A methylation with AFP was more than or equal to 20 ng / L,the sensitivity was 80. 9%,specificity was 93. 4%,AUCRASSF1 A ＋ AFPwas 0. 876（ 95% CI was among 0. 836- 0. 917）. RASSF1 A methylation positivity was associated with HCC,TNM staging and distant metastasis（ P〈0. 05）,which had no relationship with other clinical pathological characteristics. Conclusion Serum RASSF1 A methylation demonstrated a satisfactory value in the diagnosis of HBV- related HCC and could predict clinical progression.
出处
《中国卫生检验杂志》
CAS
2015年第19期3300-3302,3305,共4页
Chinese Journal of Health Laboratory Technology
关键词
肝细胞癌
RAS相关区域家族1A
甲基化
血清
Hepatocellular carcinoma
RAS association domain family 1A
Methylation
Serum
