摘要
目的探讨肺癌RAS相关区域家族1A(RASSF1A)启动子CpG岛甲基化状态和基因表达水平与肺癌发生的关系。方法用甲基化特异性PCR检测RASSF1A启动子CpG岛甲基化状态,实时定量PCR检测RASSF1A mRNA的表达水平。结果在肺癌组织中RASSF1A启动子甲基化频率为53.33%(24/45),在正常肺组织中发生甲基化的频率为13.04%(3/23)(P<0.05)。正常肺组织中RASSF1A mRNA全部表达,肺癌组织中表达缺失率为28.89%(13/45),且表达量低于正常肺组织(P<0.05);不同年龄、性别、肿瘤大小、恶性程度、肿瘤分类中其表达量无显著差异;RASSF1A甲基化与其mRNA表达水平下降密切相关。结论肺癌中RASSF1A基因启动子甲基化频率明显升高,其表达普遍下调或缺失,提示RASSF1A启动子甲基化在肺癌发生、发展中起一定作用。
Objective To investigate promoter methylation, mRNA expression of RASSF1A gene（ RAS association domain family 1 A）and their correlationship in lung carcinoma. Methods The promoter methylation of RASSF1A was detected by methylation-specific PCR （MS-PCR）, mRNA expression level was measured by real-time reverse transcription polymerase chain reaction. Results Methylation rate of RASSFIA gene was 53.33 % （24/45）in lung carcinoma tissues, 13.04% （3/23） in normal lung tissues （ Х^2 = 10. 32, P 〈 0. 05 ）. RASSF1A mRNA expressed normally in all 23 normal lung tissues,whereas, loss expression in the tissues of 28.89% （13/45）lung carcinoma, the mean level of RASSFIA mRNA was significantly lower in lung carcinoma tissues than that in normal tissues（ t = 18. 696, P 〈 0. 05）. However, it was not correlated with clinical data, such as sex, age, pathological type and tumor size. In 24 patients with the aberrant promoter methylation, 13 cases showed no expression of RASSFIA mRNA. Conclusion Frequency of RASSFIA promoter methylation is apparently higher in lung carcinoma tissues than that in normal tissues,the mean level of RASSFIA mRNA expression is also lower in lung carcinoma than in normal tissues. It signifies that RASSFIA methylation may contribute to the low expression of RASSFIA and development of lung carcinoma.
出处
《基础医学与临床》
CSCD
北大核心
2007年第9期1011-1014,共4页
Basic and Clinical Medicine
关键词
肺癌
RASSF1A
启动子
甲基化
表达
lung carcinoma
RAS association domain familyl A
promoter
ethylation
expression
