摘要
目的:在前期实验研究和"断体地龙假说"的基础上,探究断体地龙中活性成分促进创伤愈合的机制。方法:采用小鼠皮肤切除性创伤模型,制备地龙提取液,通过苏木精-伊红(HE)染色和免疫组化测定伤后创面愈合率及创面组织中血管内皮生长因子(VEGF)、碱性成纤维生长因子(b FGF)、转化生长因子(TGF-β1)的表达。结果:1.断体地龙提取液给药组与空白对照组相比创面愈合率更大,且HE染色后发现其能明显改善创面组织的愈合。2.地龙药物组VEGF、TGF-β1 2种因子的表达与生理盐水组差别不大,无统计学意义(P>0.05)。3.地龙药物组b FGF与生理盐水组相比,药物组b FGF的表达提前到达峰值,且持续时间较长;地龙药物组b FGF的表达第1天到达峰值,与对照组同一时间点阳性表达得分相比,具有显著性差异(P<0.05)。结论:地龙提取液外涂对小鼠皮肤切除性创伤的愈合有促进作用,其机制可能与VEGF、TGF-β1的表达无关,而与炎症期和增殖期创伤部位b FGF的表达增高有关系。
On the basis of pre-experiment research and the hypothesis of“amputated lumbricus”, this research was aimed to explore mechanism of active components of the amputated lumbricus to promote wound healing. Skin excision was used to establish the mice model. The amputated lumbricus extract was prepared. HE staining and immunohistochemistry techniques were used in the determination of the wound healing rate and changes of VEGF, bFGF, TGF-β1 expression during wound healing period. The results showed that compared with the blank control group, the healing rate of the amputated lumbricus extract group was better. And the HE staining showed better improvement of traumatic tissues. There was no statistic differences on the expression of VEGF and TGF-β1 between the amputated lumbricus extract group and the normal saline group （P＆gt; 0.05）. The expression of bFGF in amputated lumbricus extract group reached peak earlier than the control group and also lasted a longer time. The amputated lumbricus extract group reached peak on the first day, which had a significant difference （P 〈 0.05） compared with the control group at the same timepoint. It was concluded that the external application of amputated lumbricus extract had wound healing effect on traumatic skin of mice. Its mechanism may be irrelevant to the expression of VEGF and TGF-β1. However, it may be related to the increasing of bFGF expression in the injured regions during the inflammation stage and proliferation stage.
出处
《世界科学技术-中医药现代化》
2015年第7期1449-1453,共5页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家自然科学基金委面上项目(81373942):断体再生期的地龙促进创面修复的物质基础和作用机制研究
负责人:孙毅坤
关键词
断体地龙
创伤愈合
免疫组化
机制探究
Amputated lumbricus
wound healing
immunohistochemistry
mechanism exploration
