摘要
目的通过构建同时荷C6胶质瘤与不同炎性病变的大鼠模型，探讨18^F-FDG、11^C-MET和11^C-CHOPET／CT对肿瘤和不同炎性病变的鉴别诊断价值。方法（1）48只SD大鼠按随机数字表法随机分成6组，每组8只：1、2组构建同时荷右腹股沟C6胶质瘤、左上肢急性炎性病变模型；3、4组构建右腹股沟c6胶质瘤、左上肢慢性炎性病变模型；5、6组构建右腹股沟c6胶质瘤、左上肢炎性肉芽肿模型。（2）1、3及5组行11^C-MET和18^F-FDGPET／CT检查：2、4及6组行11^C-CHO和18^F-FDGPET／CT检查，分别计算3种显像剂的4种病变与正常肌肉的摄取比值及肿瘤选择指数（SI）。（3）显像后处死大鼠，取出肿瘤及炎性病变标本，进行常规病理HE染色和Glut-1、HIF-1d、CD98免疫组织化学检查。（4）两独立样本t检验统计分析每种显像剂在肿瘤和不同炎性病变中的代谢差异及肿瘤SI的差异。非参数秩和Kruskal-WallisH检验分析免疫组织化学指标在不同病变间的表达率差异，组间两两比较用Nemenyi法检验。结果（1）18^F-FDG、11^C-MET在C6胶质瘤中的代谢程度均高于3种炎性病变，且差异有统计学意义（t=1．425～3．901，均P〈0．05）。11^C-CHO代谢程度在各种病变之间差异均无统计学意义（t=0．031-3．901，均P〉0．05）。在肿瘤＋急性炎性病变模型中，SIMET（4．22±2．96）大于SIFDC（1．77±0．86），差异有统计学意义（t=2．717，P〈0．05）；在肿瘤＋肉芽肿模型中，SIMET（4．89±2．08）大于SIFDG（1．72±0．77），差异有统计学意义（t=2．490，P〈0．05）；在肿瘤＋慢性炎性病变模型中，SIMET（3．84±2．71）大于SIFDG（2．28±1．14），但差异无统计学意义（t=2．082，P〉0．05）。（2）不同病变的Glut-1表达率组间差异无统计学意义（H=5．940，P〉0．05），HIF-1d及CD98表达率组间差异均有统计学意义（H=17．810、26．540，均P〈0．05）。
Objective To investigate the value of 18F-FDG, 11C-MET and 11C-CHO PET/CT in the differentiation of C6 glioma from different kinds of inflammation in experimental rat models. Methods （ 1 ） A total of 48 male SD rats were randomly divided into 6 groups by the random number table ： group 1 and 2 consisted of 8 rats bearing both C6 glioma and turpentine oil-induced acute inflammation ; group 3 and 4 consisted of 8 rats bearing both C6 glioma and turpentine oil-induced chronic inflammation; group 5 and 6 consisted of 8 rats bearing both C6 glioma and BCG-induced granuloma. （2） 18F-FDG and 11 C-MET PET/CT were performed on rats of group 1, 3 and 5; 18F-FDG and 11C-CHO PET/CT were performed on rats of group 2, 4 and 6. The lesion-to-muscle ratios and tumor selectivity index （SI） were calculated. （3） Afterthe PET/CT imaging, the lesions were excised. Immunohistochemical staining was used to demonstrate the situation of Glut-l, HIF-1α and CD98. （4）Two-sample t test, Nemenyi test and nonparametric Kruskal- Wallis H test were used for statistical analyses. Results （ 1 ） XSF_FDG and 11 C-MET uptake in C6 glioma were higher than those in different inflammatory tissues（t= 1.425-3.901, all P〈0.05）. The 11C-CHO up- take among different lesions were not significant （t = 0.031-3.901, all P〉0.05）. In group 1 and 5 models, SIMET（4.22±2.96 and 4.89±2.08） was significantly higher than SIFDG（ 1.77±0.86 and 1.72±0.77; t=2.717 and 2.490, both P〈0.05） ; but in group 3 models, SIMET（ 3.84±2.71 ） was not significantly higher than SIFDG（2.28± 1.14 ; t = 2.082, P〉0.05 ）. （2） Immunohistochemical study showed that there were significant differences in the expression of HIF-1α, CD98 among different lesions （H= 17.810, 26.540, both P〈 0. 05）, and no significances of expression of Glut-1 among different lesions （H= 5.940, P〉0.05）. Nemenyi test showed that there was significant difference for CD98 expression between C6 glioma and acute inflamma- tion, C6 glioma and g
出处
《中华核医学与分子影像杂志》
北大核心
2015年第5期396-402,共7页
Chinese Journal of Nuclear Medicine and Molecular Imaging
