摘要
目的探讨^18F—FDGPET／CT显像评估马蔺子素（IR）对兔VX2肺癌模型放疗增敏作用的可行性。方法建立24只兔VX2肺癌模型，按随机数字表法分为A（单纯放疗）组、B（IR＋放疗）组及c（对照）组，每组各8只。各组分别于治疗前、治疗后24h及治疗后1周行^18F—FDGPET／CT显像，观察3组肿瘤部位延迟显像SUVmax的变化。显像完成后每组处死2只荷瘤兔，取出肿瘤组织，行HE染色观察病理改变。数据分析采用配对t检验、单因素方差分析及Kaplan—Meier生存分析。结果全部兔VX2肺肿瘤放疗前PET／CT显像SUV-为2．200±0．761，延迟显像SUVmax为3．162±0．833，差异有统计学意义（t=-5．582，P〈0．01）。放疗后24hA、B、C组延迟显像SUV-分别为2．614±0．654、2．349±0．869和5．663±1．144，3组与治疗前延迟显像SUV-差异均有统计学意义（t=2．527、3．620和11．011，均P〈0．05）；放疗后1周时A、B、C组延迟显像SUV-分别为3．625±1．064、3．058±0．850及7．424±1．751，各组间差异均有统计学意义（tA：B=2．652、tA：C=3．799和tB：C=4．566，均P〈0．05），病理结果显示B组肿瘤细胞明显少于A、C组，与PET／CT结果相符。A、B、C3组生存时间分别为（62．375±4．534）、（69．000±4．660）和（54．125±5．276）d，Kaplan—Meier生存分析显示B组生存率较A组及C组明显增高（Log—rank检验,X2=7．355和16．943，均P〈0．01）。结论^18 F-FDGPET／CT可评估放疗早期疗效及马蔺子素对放疗的增敏作用。
Objective To evaluate the value of ^18F-FDG PET/CT in assessing radiosensitivity enhancement by irisquinone （IR） on rabbit xenografted VX2 lung tumor models. Methods Twenty-four tumor-bearing rabbits were randomly divided into 3 groups （8 rabbits/group）： group A with radiotherapy alone, group B with combined radiotherapy and IR, and group C without radiotherapy （the control group）. ^18F- FDG PET/CT imaging was performed before radiotherapy and 24 h and one week after radiotherapy. The tumor SUVmax on delayed imaging was calculated in all rabbits. Two rabbits in each group were sacrificed after PET/CT imaging. HE staining was used to assess the differences in cancer cells among groups. Paired t test, one-way analysis of variance and Kaplan-Meier analysis were performed to analyze the data using SPSS 13.0. Results Before radiotherapy, the tumor SUVmax of all the 24 rabbits on standard and delayed imaging were 2. 200 ＋ 0. 761 and 3. 162±0.833 （ t = - 5. 582, P 〈 0.01 ）. At 24 h post-radiotherapy, the delayed SUVmax of groups A, B and C were 2. 614 ± 0. 654, 2. 349 ± 0.869 and 5. 663 ± 1. 144, respectively. The differences between pre-radiotherapy and 24 h post-radiotherapy were statistically significant in all three groups （t =2. 527, 3. 620, 11. 011, all P 〈0.05）. One week after radiotherapy, the delayed SUVmax of groups A, B and C were 3. 625 ：t= 1. 064, 3. 058 ± 0,850 and 7. 424 ± 1.751, respectively. The differences among groups A, B and C were statistically significant （ tA： B = 2. 652, tA：C = 3. 799, tB： c = 4. 366, all P 〈 0. 05 ）. The cancer cells of group B were fewer than those of groups A and C by pathological findings,which was consistent with ^18F-FDG PET/CT results. The survival times of groups A, B and C were （62. 375 ± 4. 534 ）, （69. 000 ±4. 660） and （54. 125 ± 5. 276） d, respectively. Kaplan-Meier survival curves revealed better survival of group B as compared to groups A and C, respectively （ Log-rank, X2 = 7. 355, 16. 943, both P 〈
出处
《中华核医学与分子影像杂志》
CSCD
北大核心
2013年第2期150-153,共4页
Chinese Journal of Nuclear Medicine and Molecular Imaging
基金
安徽省科技计划项目（10021303025）
