摘要
目的 探讨环氧合酶-2（COX-2）基因多态性与非小细胞肺癌（NSCLC）易感性的关系.方法 我们收集了42例非小细胞肺癌患者和50例健康体检者的空腹静脉血,使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析两组COX-2基因多态性.结果 发现COX-2 rs689466的基因型AA在患病组的分布频率显著高于健康对照组（P＜0.05）,AA基因型比GG基因型患NSCLC的风险高出4.67倍.COX-2 rs5275的基因型CC在这两组中的分布有差异（P＜0.05）,CC基因型比TT基因型患NSCLC的风险高出7.02倍.对性别、年龄、吸烟和饮酒因素修正后,COX-2 rs689466的基因型AA和COX-2 rs5275的基因型CC依旧会增高NSCLC的易感性.对两个单核苷酸多态性（SNP）等位基因的连锁不平衡和单体型分析之后发现,患病组中A-C单体型的分布频率较对照组高,且两组比较差异有统计学意义（P＜0.05）.对性别、年龄、吸烟和饮酒因素修正后,两组A-C单体型的分布频率差异有统计学意义（P＜0.05）.结论 COX-2 rs689466和rs5275基因多态性与NSCLC的易感性相关,且A-C是NSCLC的易感单体型.
Objective To explore the association between cyclooxygenase-2 （COX-2） polymorphisms and non-small cell lung cancer （NSCLC） susceptibility.Methods We collected fasting peripheral venous blood from 42 cases with NSCLC and 50 healthy controls through physical examinations,and used polymerase chain reaction-restricted fragment length polymorphism （PCR-RFLP） to analyze COX-2 polymorphisms of two groups.Results The distribution frequency of genotype AA of COX-2 rs689466 in case group was significant higher than that in control group （P ＜ 0.05）.Carriers with AA genotype were 4.67 times at risk of NSCLC than those with GG genotype.The distribution of genotype CC of COX-2 rs5275 was different between two groups （P ＜ 0.05）,and carriers with genotype CC were at 7.02 times higher risk of NSCLC than those with genotype TT.After corrected by sex,gender,smoking and drinking factors,AA genotype of COX-2 rs689466 and CC genotype of COX-2 rs5275 still contributed to increased risk of NSCLC.After analyzed of linkage disequilibrium （LD） and haplotypes of alleles in two SNPs,the distribution frequency of A-C haplotype in case group was higher than that in control group,with significant difference between two groups （P ＜ 0.05）.After corrected by sex,gender,smoking and drinking factors,statistical difference was still found in the total distribution of A-C haplotype between two groups （P ＜ 0.05）.Conclusion COX-2 rs689466 and rs5275 polymorphisms may be related to NSCLC susceptibility.And A-C haplotype might be a susceptibility haplotype for NSCLC.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2015年第9期2264-2266,共3页
Chinese Journal of Experimental Surgery
关键词
环氧合酶-2
多态性
易感性
非小细胞肺癌
Cyclooxygenase-2
Polymorphism
Susceptibility
Non-small cell lung cancer
