摘要
目的探讨COX-2基因的序列变异是否与前列腺癌的发病风险有关。方法在基于医院的病例/对照研究中,采用PCR-RFLP研究250例前列腺癌患者与195例健康对照组COX-2-765 G>C(rs20417)和+8473 T>C(rs5275)启动子多态性与前列腺癌易感性的关系。结果载变异等位基因C的COX-2-765G>C的多态性与1.7倍增加的前列腺癌的发病风险相关。COX-2+8473 T>C基因型CC的多态性被认为与整体前列腺癌较高的发病风险显著相关。组合基因型(TC+CC)也证明了一个1.5倍的显著前列腺癌发病风险。C-C的属性与显著增高的前列腺癌发病风险有关。根据临床病理分级发现病例的层理与前列腺癌的风险无关。结论本研究结果表明COX-2-765 G>C和t8473 T>C的多态性和C-C的属性为前列腺癌的危险因子。然而,需要基于大规模人群更进一步的研究来证实该研究。
[ objective ] We examined whether sequence variants in the COX-2 gene were associated with prostatecancer （PCa） risk. [Methods] In a hospital-based case/control study, 195 subjects with PCa and 250 healthy con-trois were investigated for the association of COX-2765 G〉C （rs20417） and t8473 T〉C （rs5275） promoter polymor-phism with PCa susceptibility using the polymerase chain reaction restriction fragment length polymorphism （PCR-RFLP） method. [ Results ] Variant allele ＇ C＇ carriers of COX-2 765 G〉C polymorphism were associated with a 1.7-fold increased risk for PCa （ P =0.014; OR--1.72）. The variant genotype CC of COX-2 ＋8473 T〉C polymorphism wasfound to be significantly associated with the overall higher risk of PCa （P =0.042; OR=1.79）. Combined genotype （TCt CC） also demonstrated a 1.5-fold significant risk with PCa （P =0.038; OR=1.46）. The diplotype C-C was observedto be associated with a significant increase in PCa risk （Bonferroni corrected P value, Pc =0.007; OR=4.33）. Stratifi-cation of cases based on clinical pathological grade of tumors revealed no association with PCa risk. [ Conclusion ]Our findings suggest COX-2 765G〉C and ＋8473 T〉C polymorphism and diplotype C-C to be a risk factor for PCa.However, further validation in large population-based studies is needed to confirm the finding.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2014年第15期39-43,共5页
China Journal of Modern Medicine
