摘要
目的:研究COX-2-899G>C,COX-2 codon587G>A基因多态性在胃癌高发区甘肃河西地区健康人群与胃癌患者的分布,检测幽门螺旋杆菌(H pylori)在上述人群中的感染情况,探讨COX-2-899G>C,COX-2 codon587G>A基因多态性以及H pylori感染与河西地区胃癌发生的关系.方法:采用PCR-TaqMan探针法检测甘肃河西地区健康人群和胃癌患者COX-2-899G>C,COX-2 codon587G>A的基因多态性,用Warhin-starry染色法检测本研究对象的Hpylori感染率.结果:COX-2-899G>C分为G/G,G/C,C/C三种基因型,其频率在胃癌患者中分别为72.9%,21.4%,5.7%;在普通人群中分别为84.0%,12.8%,3.2%.与G/G基因型相比,COX-2-899*C基因携带者患胃癌的风险增加(OR=1.956,95%CI:1.067-3.586).COX-2 codon587G>A三种基因型为G/G,G/A,A/A,其频率在胃癌患者中分别为86.4%,11.4%,2.2%;在健康人群中分别为89.6%,9.6%,0.8%.COX-2 codon587G>A三种基因型在胃癌组和健康对照组间无显著性差别.Hpylori感染率在胃癌组和对照组分别为68.6%,50.4%,两组间具有显著差异(P=0.003).分层分析提示COX-2-899*C基因携带者若同时伴有H pylori感染,其患胃癌的风险明显增加(OR=2.471,95%CI:1.076-5.675).结论:COX-2-899G>C的C等位基因增加我国胃癌高发区甘肃河西地区胃癌发病的风险,而且与H pylori感染对胃癌的发病有一定的协同作用;COX-2 codon587G>A基因多态性与甘肃河西地区胃癌的易感性无关.
AIM： To investigate the relationship among cyclooxygenase-2 （COX-2） polymorphisms （COX-2-899G〉C and COX-2 codon587G〉A）, H pylori infection and susceptibility to gastric cancer in high-incidence Hexi area of Gansu Province in China. METHODS： Blood samples were collected from 140 patients with gastric carcinoma and 125 normal persons in Hexi area of Gansu Province. Genomic DNA was extracted by phenol chloroform method and polymorphisms of COX-2-899G〉C （G to C） and codon 587G〉A（G to A） were genotyped by PCR-TaqMan. For detection of H pylori infection, Warhin-starry staining was used. RESULTS： Three kinds of COX-2-899G〉C genotypes were found, including GG, GC and CC. Their frequencies in gastric cancer patients were 72.9%, 21.4%, 5.7% and their frequencies in the normal controls were 84.0%, 12.8%, 3.2%, respectively. COX-2-899C carriers had an increased risk of gastric carcinoma （OR = 1.956, 95% CI： 1.067-3.586）. COX-2 587codonG〉A included three genotypes： G/G, G/A and A/A. Their frequencies in the cancer patients were 86.4%, 11.4%, 2.2%, and in the normal controls were 89.6%, 9.6%, 0.8%, respectively. However, No significant differences of COX-2 587codon G〉A polymorphisms were observed between the cancer patients and the normal controls in each genotype. The rate of H pylori infection was significantly higher in the cancer patients than that in the healthy control （68.6% vs 50.4%, P = 0.003）. Stratification analysis showed that COX-2 -899C carrier with H pylori infection had a higher risk for gastric cancer （OR = 2.471, 95% CI： 1.076-5.675）.
CONCLUSION： COX-2 -899C genotype may increase the susceptibility to gastric cancer in highincidence Hexi area of Gansu province in China. In addition, H pylori infection and COX-2 -899C play a synergic role in gastric cancer pathogenesis. However, COX-2 codon 587G〉A has no relation with gastric cancer in this area.
出处
《世界华人消化杂志》
CAS
北大核心
2008年第21期2364-2370,共7页
World Chinese Journal of Digestology
