摘要
以玉米自交系昌7-2为材料,采用改良CTAB法提取基因组DNA,分析不同保存和研磨方法等对样品提取效果的影响。通过A260/A280、琼脂糖凝胶电泳、PCR扩增,对提取的基因组DNA进行分析鉴定。结果表明,CTAB缓冲液保存叶片并在CTAB缓冲液中研磨叶片,得到的DNA效果最佳,同时这种方法也比较经济安全。
Effects of various preservation and grinding methods, 18 treatment combinations, for extracting total DNA with modified CTAB method from the maize inbred line Chang 7-2 were studied. The quality and quantity of extracted DNA was examined using the A260/A280 value, the agarose gel electrophoresis, and PCR amplification. The results showed that CTAB buffer preservation and grinding leaves was the best way to extract DNA, and this treatment was relatively economical and safe.
出处
《山西农业科学》
2015年第9期1089-1092,共4页
Journal of Shanxi Agricultural Sciences
基金
国家转基因生物新品种培育重大专项(2013ZX08003-001)
