摘要
为了从DNA水平上快速鉴定Glu-A1位点编码的1Ax2*优质亚基,建立了稳定的检测1Ax2*优质亚基基因的PCR扩增体系。通过该体系,1Ax2*优质亚基基因扩增出2652bp特异片段,而其他亚基基因则不能扩增出该片段。验证材料的PCR鉴定结果与SDS-PAGE电泳结果一致,表明利用该体系鉴定1Ax2*亚基是可行的。利用此体系鉴定了44份外引小麦品种(系)的谷蛋白Glu-A1位点,有24个品种(系)含有1Ax2*亚基,1Ax2*亚基出现频率为54.5%。
In order to identify the 1Ax2^＊ subunit of high molecular weigh （HMW） glutenin coded by Glu-A1 locus at the DNA level, we constructed a stable ASPCR amplified system of detecting 1Ax2^＊ subunit gene, which 1Ax2^＊ subunit gene could be amplified as a 2 652bp fragment, while other sub- unit genes on GluA1 locus could not be amplified. The result of AS-PCR identification was accordant with SDS-PAGE identification for testing varieties, showed that the AS-PCR system can be used in detecting of 1Ax2^＊ subunit. We used this AS-PCR method to detect 44 foreign wheat varieties （lines）. The results showed that 24 varieties（lines）（54. 5%） had 1Ax2^＊ subunit and 20 varieties （lines） had not.
出处
《麦类作物学报》
CAS
CSCD
北大核心
2006年第3期75-77,共3页
Journal of Triticeae Crops
基金
农业结构调整重大技术研究专项(04-02-05B)
科技部农业科技成果转化资金项目(2004-309)。
