摘要
为发掘新的种质资源,利用SDS-PAGE方法对144份CIMMYT小麦材料的高分子量谷蛋白亚基（HMW-GS）进行了分析。结果表明：在供试材料中共检测出9种HWM-GS类型和14种亚基组合类型。Glu-A1位点具有1、2＊与null（N）共3种类型,亚基2＊占优势,占55.56%;Glu-B1位点具有7＋9、17＋18、7、7＋8共4种类型,7＋9为优势亚基,占59.72%;Glu-D1位点具有5＋10、2＋12这2种类型,5＋10占优势,占79.86%。优势亚基组合类型为2＊、7＋9、5＋10,占41.67%。144份材料的高分子谷蛋白亚基品质评分变幅为4~10分,平均为8.54分。在优质亚基中,Glu-A1位点具有优质亚基（l和2＊）的频率为86.8%;Glu-B1位点具有优质亚基（7＋8和17＋18）的频率为30.56%;Glu-D1位点具有优质亚基5＋10的的频率为79.86%。本试验同时筛选出一大批含优质亚基的育种资源材料。今后小麦品质育种中,还应加强对具有优质亚基2＊、17＋18的利用。
In order to explore new wheat germplasm for wheat quality breeding,the high-molecular-weight subunits（ HMW-GS） of 144 CIMMYT wheat materials were analyzed by SDS-PAGE. The results showed that 9 subunits and 14 subunits combinations within the 144 materials were detected. Three types（ 1,2＊and null） were detected on Glu-A1 loci,and 2＊subunit was the major type with frequency of 55.56 %; Four types（ 7 ＋ 9,17 ＋ 18,7 and 7 ＋ 8） were detected on Glu-B1 loci,and 7 ＋ 9 subunit was the major type with frequency of 59.72 %; Two types（ 5 ＋ 10 and 2 ＋ 12） were detected on Glu-D1 loci,and 5 ＋ 10 subunit was the major type with frequency of 79. 86 %. The dominant subunits combination was 2＊,7 ＋ 9,5 ＋ 10 with frequency of 41. 67 %. The quality scores of the 144 wheat materials were between 4 and 10 points,and the mean quality score was 8. 54. The frequencies of the good subunits were 86. 8 %（ l and 2＊）,30. 56 %（ 7＋ 8 and 17 ＋ 18）,79. 86 %（ 5 ＋ 10）,respectively. A large number of breeding resource materials with good quality were also obtained in this study. Therefore,the utilization of wheat genetic resources with good subunits（ 2＊and 17 ＋ 18） should be strengthened in future quality breeding program.
出处
《西南农业学报》
CSCD
北大核心
2015年第4期1470-1474,共5页
Southwest China Journal of Agricultural Sciences
基金
现代农业产业技术体系建设专项资金(CARS-03)
四川省"十二五"育种攻关资助项目(2011YZGG-3)
国家现代农业产业技术体系四川省麦类创新团队项目资助
