摘要
目的建立鹿鞭及其伪品随机扩增DNA多态性(RAPD)鉴定方法,为鹿鞭的鉴别提供可靠依据.方法采用柱层析法从鹿鞭及其伪品中提取线粒体DNA并进行随机DNA多态性扩增.结果正品鹿鞭(梅花鹿鞭和马鹿鞭)与其常见伪品(牛鞭)线粒体DNA随机扩增产物的电泳图谱有明显区别,条带数量和位置均不同,说明本方法可以有效区分正品鹿鞭与其常见伪品.结论 RAPD技术可为鹿鞭及其伪品的鉴别提供一种简单、有效、可信度高的方法.
Objective To establish the identification method of Penis Cervi from its adulterants by RAPD technology, and to provide a reliable evidence for Penis Cervi identification. Method Column chromatography method was used to extract and purify mt DNAs from Penis Cervi and its adulterants, and the DNA fragments were amplified by RAPD technology. Results The results showed that there was a great difference in the number and brightness of the stripes of RAPD products between Penis Cervi （ C, ervus nippon Temminck whip and Cervus elaphus Linnaeus whip） and its adulterants （Bull whip）. Conclusion The technology of RAPD could be a simple, effective and reliable method for the identification of Penis Cervi from its adulterants.
出处
《北华大学学报（自然科学版）》
CAS
2015年第5期605-608,共4页
Journal of Beihua University（Natural Science）
基金
吉林省教育厅科学技术研究项目(2012143
2014212)
吉林省战略性新兴产业和高新技术产业发展专项基金(2013G030
2013G019)
关键词
梅花鹿鞭
马鹿鞭
牛鞭
RAPD
指纹图谱
Gervus nippon Temminck whip
cervus elaphus Linnaeus whip
bull whip
RAPD
fingerprint
