摘要
本研究根据猪嵴病毒(porcine kobuvirus,PKV)VP0基因序列特征设计特异性扩增引物,采用RT-PCR方法扩增猪嵴病毒VP0基因全长编码区。将特异性扩增目的片段克隆后进行序列测定,将结果进行拼接后获得猪嵴病毒VP0基因全长并进行相关生物信息学分析。所扩增的目的片段编码有完整的VP0基因开放阅读框,全长为1 098bp,编码有366个氨基酸,理论等电点为6.71,理论分子质量为38.489ku,不稳定系数为34.65,最大疏水指数为2.622,最小疏水指数为-2.122。将获得的VP0基因和GenBank中的猪嵴病毒代表株VP0基因序列进行核苷酸同源性比对和遗传进化分析,其与HNXX-4核苷酸同源性最高,为89.1%,与S-1-HUN核苷酸同源性最低,为81.1%。从遗传进化上看,猪嵴病毒VP0基因在遗传进化上呈两个独立的基因亚群,猪嵴病毒中国分离株在两个遗传基因亚群上均有分布。
In this study,specific primers were designed according to VP0 gene of porcine kobuvirus（PKV）,and the full-length coding region of VP0 gene was amplified by RT-PCR method and sequenced,then bioinformatics analysis were conducted to investigate the structure and function of the porcine kubovirus VP0 gene.The results showed that the porcine kobuvirus VP0 gene was1 098 bp in length,coding an open reading frame（ORF）with 366 amino acids.The isoelectric point,molecular weight and instability index of porcine kubovirus VP0 were 6.71,38.489 ku,34.65,respectively.The maximum and minimum hydrophobicity were 2.622and-2.122,respectively.Compared with the porcine kubovirus VP0 genes published previously in GenBank,the sequenced gene shared the highest homology with HNXX-4strain,which was 89.1%;And shared the lowest homology with S-1-HUN strain,which was 81.1%.The porcine kubovirus VP0 gene shared two different phylogenetic genotype branches,and the Chinese porcine kubovirus isolates distributed in the two phylogenetic genotype branches.
出处
《中国畜牧兽医》
CAS
北大核心
2015年第9期2303-2307,共5页
China Animal Husbandry & Veterinary Medicine
基金
福建省属公益类科研专项(2014R1102
2013R1025-8)
