摘要
为了对猪Kobu病毒进行快速诊断及流行病学监测,根据GenBank中公布的9条猪Kobu病毒基因组全序列,在3CD非结构蛋白保守区域设计并合成了2对特异性引物,建立了猪Kobu病毒的巢式RT-PCR检测方法,并用该方法对猪Kobu病毒DX株动物回归试验发病的25头仔猪的组织病料以及送检的72份临床样本进行检测。结果表明,使用该方法对25头发病猪内脏病料的阳性检出率为100%;对送检的72份临床样品的阳性检出率为87.5%(63/72)。该方法特异性强、灵敏度高、重复性好,适用于猪Kobu病毒的临床快速诊断及流行病学监测。
In order to establish a nested RT - PCR assay for diagnosis of porcine kobuviruses, two pairs of specific primers were designed and synthesized in the conserved region of 3CD non -structural proteins according to 9 porcine kobuviruses complete genome in GenBank. The assay was used to detect samples of 25 infected piglets with strain PKV - DX and 72 clinical samples from areas of Beijing, Xinjiang, and Inner Mongolia. The positive rate of 25 samples was 100%, and that of 72 clinical samples was 87.5%. The nested RT -PCR assay with high specificity, sensitivity and repeatability can be applied to the clinical rapid detection and eDidemiolozical surveillance.
出处
《中国兽药杂志》
2013年第8期5-8,共4页
Chinese Journal of Veterinary Drug
