摘要
采用RT-PCR方法对2009—2011年山西省分离的5株猪繁殖与呼吸综合征病毒（Porcine reproductive and respiratory syndrome virus,PRRSV）的ORF5和Nsp2（2503～3269nt）基因进行克隆和测序，并对其基因序列和推导的氨基酸序列与国内外毒株进行了同源性分析。序列分析结果显示，5株分离株Nsp2基因与国内分离的PRRSV变异株（JXA1、HuN、HUN4、HUB1）的序列同源性最高，为96．8％～98．2％，且缺失位置一致，均存在2个位点30个氨基酸缺失；ORF5基因大小为603bp，编码200个氨基酸，第13、151位均为具有强毒特性的精氨酸（R），137位为丝氨酸（S），表明这5株均为野毒株，与国内分离的PRRSV变异株（JXA1、HuN、HUN4、HUB1）毒株的序列同源性最高，为96．5％～98．0％。结果表明，山西省内目前流行的PRRSV为Nsp2缺失30个氨基酸的变异毒株。
ORF5 and Nsp2 genes of 5 PRRSV strains isolated from Shanxi province were amplified by RT-PCR,cloned and sequenced. The obtained sequences and the deduced amino acid were analyzed and compared with the other published PRRSV strains. Sequence comparision showed that Nsp2 genes of the 5 isolated strains were up to 96.8%-98.2% homology with JXA1, HuN, HUN4 and HUB1 isolated from China and had the same 30 amino acid deletion. The ORF5 genes were 603 bp in length and consist of 200 amino acids and the site of 13 and 151 were Arginine （R） which were related to virulent strains,and the site of 137 was serine （S） indicating that the 5 isolated strains were wild strains. The nucleotide homology are 96.5;/0T98. 0% comparing with JXA1,HuN, HUN4 and HUB1 isolated from China. The results Showed the prevalent PRRSV strains in Shanxi were the Nsp2 gene deleted 30 amino acid,which laid the foundation for the scientific prevention and control of PRRS.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2014年第1期1-5,共5页
Chinese Journal of Veterinary Science
基金
山西省科技攻关项目(20080311036-1)
山西省青年科技研究基金资助项目(2012021028-1)
