摘要
为了快速鉴别诊断高致病性猪繁殖与呼吸综合症,参照GeneBank已发表的猪繁殖与呼吸综合征病毒(PRRSV)的Nsp2基因,设计了1对引物,建立了一种RT-PCR检测方法.该方法可以区分高致病性PRRSV毒株与经典PRRSV弱毒疫苗株,扩增PCV2、PRV、PPV和CSFV等均为阴性.该方法敏感性较高,可以检测到0.2 ng的病毒核酸.
A pair of primers based on Nsp2 gene of porcine reproductive and respiratory syndrome virus （PRRSV） was designed and a method of RT-PCR was developed to distinguish the highly pathogenic PRRSV from the classic attenuated vaccine strain. The method was sensitive to detect as low as 0. 2 ng viral genome. PCV2, PRV, PPV and CSFV were negative in their reactions.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2008年第3期85-87,共3页
Journal of South China Agricultural University
基金
广东省科技计划项目(2007A020300006-4)
教育部创新团队项目(IRT0723)
广东省动物防疫检疫专项经费(粤财农2007-479)
