摘要
目的：研究HIV、HBV共感染患者病毒载量与外周T淋巴细胞亚群的相关性。方法选取2011年1月-2014年1月就诊于乐清市血站门诊和住院部未经高效抗逆转录病毒治疗的HIV感染患者1230例为单纯感染组和HIV、HBV共感染患者148例为共感染组。采用RT-PCR、荧光定量PCR和流式细胞仪进行HIV、HBV病毒载量和T淋巴细胞亚群计数检测，采用全自动生化分析仪测定患者相关生化指标，比较两组的检测结果。分析HIV、HBV共感染患者病毒载量与T淋巴细胞亚群的相关性。结果两组患者丙氨酸氨基转移酶、天冬氨酸氨基转移酶、总蛋白、白蛋白、总胆红素水平和HIV RNA载量比较，差异均有高度统计学意义（P〈0.01）。两组患者CD4＋、CD8＋、CD3＋T淋巴细胞亚群计数和CD4＋/CD8＋比较，差异有高度统计学意义（P〈0.01）。 HIV RNA载量与HBV DNA载量呈正相关（r=0.471）；CD4＋/CD8＋与HBV DNA载量呈负相关（r=-0.412）；CD4＋、CD4＋/CD8＋与HIV RNA载量呈负相关（r=-0.841、-0.583）。结论 HBV可能存在促进HIV复制的功能，患者的肝损伤和免疫损伤较单纯HIV感染更为严重，在临床上应引起重视。
Objective To investigate the correlation between viral load and peripheral T lymphocyte subset in patients with HIV and HBV coinfection. Methods From January 2011 to January 2014 in Yueqing Blood Supply Center of Out-patient and Inpatient Department, 1230 cases of untreated HIV infected patients with highly active antiretroviral thera-py were selected as simple infection group, 148 patients with HIV and HBV coinfection were selected as coinfection group. RT-PCR, fluorescent quantitative PCR and flow cytometry were adopted to detect the HIV and HBC viral load and T lymphocyte subsets count. Fully automatic biochemical analyzer was adopted to detect related biochemical index. The detection results between two groups were compared. The correlations between viral load and T lymphocyte subsets count in HIV and HBV coinfection patients were analyzed. Results ALT, AST, TP, ALB, TBIL level and HIV RNA load between two groups was compared respectively, there were significantly statistical differences （P〈 0.01）. CD4＋, CD8＋, CD3＋T lymphocyte subsets count and CD4＋/CD8＋was compared respectively, there were significantly statistical differences （P〈0.01）.There was a positive correlation of HIV RNA load and HBV DNA load in coinfection patients （r=0.471）. CD4＋/CD8＋was negatively correlate with HBV DNA load （r=-0.412）. CD4＋, CD4＋/CD8＋were negatively corre-late with HIV RNA load （r =-0.841,-0.583）. Conclusion HBV may has promoting HIV RNA replication effect which makes a worse liver damage and immune system damage in coinfection patients than HIV infection along patients, should pay attention to in clinic.
出处
《中国医药导报》
CAS
2015年第26期103-106,共4页
China Medical Herald
基金
浙江省乐清市科技计划项目(2014Y003)
