摘要
目的:探讨二苯乙烯苷抗动脉粥样硬化的可能机制。方法:载脂蛋白E基因敲除小鼠(apo E-/-小鼠)随机分为3组(n=6),模型对照组(高脂饮食),二苯乙烯苷小剂量组(高脂饮食+二苯乙烯苷20 mg/kg),二苯乙烯苷大剂量组组(高脂饮食+二苯乙烯苷40 mg/kg),人工饲养12周后行小鼠主动脉弓HE染色,确认模型对照组斑块形成。另外选用6只C57BL/6J小鼠作为正常对照组。根据分组,给予药物干预4周,给药期间全部换用普通饲料。实验结束前,取血清,并取主动脉。HE检测粥样斑块;生化法检测血脂;elisa法检测血清中oxLDL的浓度;western blot法检测、细胞间黏附分子-1(ICAM1-1)、白介素-1(IL-1)、肿瘤坏死因子-α(TNF-α)、凝集素样氧化型低密度脂蛋白受体(LOX-1)的表达。结果:(1)HE染色结果显示:空白对照组主动脉内膜未见斑块;模型对对照组斑块明显;二苯乙烯苷组斑块面积显著缩小。(2)模型对照组血脂上升,二苯乙烯苷组血脂有一定降低。(3)模型组血管组织ICAM1、IL-1、TNF-α、LOX-1表达上调,二苯乙烯苷组ICAM1、IL-1、TNF-α、LOX-1表达显著下调。结论:二苯乙烯苷能够通过降低血脂,抑制抑制ox-LDL与LOX-1,调整炎性介质的表达发挥其抗动脉粥样硬化的作用。
Objective： To investigate the effect of stibene glucoside on ELAM1,ICAM1,IL-1,TNF-α and LOX-1in high fat-fed apo E-/-mice. Methods： The apo E-/-mice were randomly divided into 3 groups（n = 6） ： model group（high fat diet）,stibene glucoside low dose group（high-fat diet ＋ 20 mg/kg stibene glucoside） and stibene glucoside high dose group（high-fat diet ＋ 40 mg/kg stibene glucoside）. After 12 weeks,aortic arch was observed by HE staining to confirm the model group＇s plaque formation. Another optional six C57 BL/6J mice were as normal controls. According to the packet,mice were given medicine for four weeks and during the administration all used an ordinary feed.Before the end of the experiment,the serum and aorta were collected. TG,TC and LDL-C were measured. Concentrations of ox-LDL were measured by ELISA assay. Aortic sections were stained with hematoxylin and eosin and observed under microscope. Western blot was performed to visualize the expressions of vascular ICAM1,IL-1,TNF-αand LOX-1. Results：（1） HE staining showed that the control group had no aortic intimal plaque and the model had obvious plaque; stibene glucoside significantly reduced the plaque size.（2） Lipids increased in model group and stibene glucoside significantly reduced lipids.（3） The vascular tissue factors such as ICAM1,IL-1,TNF-α and LOX-1 in model group were up-regulated,. ICAM1,IL-1,TNF-αand LOX-1 expressions were significantly reduced in stibene glucoside group. Conclusions：The stibene glucoside can reduce plaque size by adjusting ox-LDL and the expression of inflammatory mediators.
出处
《中华中医药学刊》
CAS
北大核心
2015年第9期2086-2088,I0002,共4页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金项目(81302902)
关键词
动脉粥样硬化
二苯乙烯苷
血脂
炎症因子
atherosclerosis
stibene glucoside
ox-LDL
inflammatory cytokines
