摘要
目的探索二苯乙烯苷对HepG2细胞胆固醇含量的影响及其作用机制。方法将细胞随机分为正常组、模型组(ox-LDL 50 mg/L)、A组(ox-LDL 50 mg/L+二苯乙烯苷250μmol/L)、B组(ox-LDL50 mg/L+二苯乙烯苷188μmol/L)、C组(ox-LDL 50 mg/L+二苯乙烯苷125μmol/L)及D组(ox-LDL50 mg/L+二苯乙烯苷62.5μmol/L)。细胞处理48 h后,采用油红O脂肪染色法观察、胆固醇测定试剂盒测定各组胆固醇的含量并用逆转录聚合酶链反应检测肝癌HepG2细胞ABCG1、ABCA1及SR-BI的表达水平。结果正常组、A、B、C和D组细胞内总胆固醇(TC)、细胞内游离胆固醇(FC)含量均低于模型组(P <0.05),同时A、B、C和D组上清TC和FC含量较正常组升高(P<0.05)。二苯乙烯苷能够降低HepG2细胞内胆固醇含量,增加胆固醇外流率(P<0.05),其中二苯乙烯苷浓度为125μmol/L时达最高值。模型组细胞ABCA1 mRNA表达水平较正常组下降(P<0.05)。A、B、C和D组ABCA1、ABCG1和SR-BI mRNA表达水平较模型组上升(P<0.05)。结论二苯乙烯苷能够降低HepG2细胞胆固醇的含量,其机制可能是通过上调ABCA1、ABCG1和SR-BI的表达水平而增加胆固醇外流。
Objective To explore the influence and mechanism of stilbene glycoside on cholesterol content in HepG2 cells. Methods The cells were randomly divided into six groups: normal group, model group (ox-LDL50 mg/L) and two styrene glycoside group A (ox-LDL50 mg/L+250μmol/L), group B (ox-LDL50 mg/L+188μmol/L), group C (ox- LDL50 mg/L+125μmol/L), group D (ox-LDL50 mg/L+62.5μmol/L). After 48 h treatment, the cholesterol content of each group was observed by oil red staining and determined by cholesterol test kit, and the expression levels of ABCG1, ABCA1 and SR-BI genes in HepG2 cells of liver cancer were detected by RT-PCR technology. Results The intracellular total cholesterol (TC) and intracellular free cholesterol (FC) contents in normal group, A, B, C and D groups were lower than those in model group (P < 0.05), while the supernatant TC and FC contents in A, B, C and D groups were higher than those in normal group (P < 0.05). Stilbene glycoside can reduce the cholesterol content in HepG2 cells and increase the cholesterol outflow rate (P < 0.05), and the highest concentration of stilbene glycoside is 125 mmol/L. The expression of ABCA1 in model group was lower than that in normal group (P < 0.05). The expression levels of ABCA1, ABCG1 and SR-BI in group A, B, C and D were higher than those in model group (P < 0.05). Conclusions Stilbene glycoside can reduce the cholesterol content of HepG2 cells, and its mechanism may be increase the cholesterol outflow by up-regulate the expression levels of ABCA1, ABCG1 and SR-BI.
作者
林昶
李玉平
齐建彤
郑曙光
朱璨
柴艺汇
徐昌君
王和生
杨长福
Chang Lin;Yu-ping Li;Jian-tong Qi;Shu-guang Zheng;Can Zhu;Yi-hui Chai;Chang-jun Xu;He-sheng Wang;Chang-fu Yang(Guizhou University of Traditional Chinese Medicine,Guiyang,Guizhou 550002,China)
出处
《中国现代医学杂志》
CAS
2019年第14期7-12,共6页
China Journal of Modern Medicine
基金
贵州省国内一流建设学科项目(No:GNYL[2017]008)
贵州省普通高等学校治未病工程研究中心[No:黔教合KY(2016)013]
