摘要
目的建立超高效液相色谱法（ultra performance liquid chromatography,UPLC）分离测定丹七片中三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1含量。方法采用ACQUITY UPLC HSS T3（2.1 mm×50 mm,1.8μm）色谱柱,柱温30℃,以乙腈（A）-水（B）为流动相,梯度洗脱,流速0.3 m L/min,检测波长203 nm,进样体积2μL。结果三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1分别在0.0300~0.3000,0.1201~1.2009,0.1200~1.1997μg范围内线性关系良好,方法重复性及回收率均符合要求,平均加样回收率（n=6）分别为100.43%、100.65%、98.74%,RSD值分别为1.51%、1.30%、1.03%。按测定的方法测定市售2种丹七片的含量相近。结论该方法简便、高效、准确、重复性好,分析速度提高近8倍,可以用于测定丹七片中三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1的含量。
Objective To establish a method to determine the content of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 in Danqi tablets by ultra performance liquid chromatography（ UPLC）. Methods ACQUITY UPLC HSS T3（ 2. 1 mm × 50 mm,1. 8 μm） column was used with mobile phase consisted of acetonitrile（ A）-water（ B） by gradient elution at a flow rate of 0. 3 m L / min and a column temperature of 30 ℃,the wavelength of detector was 203 nm,the injection volume was 2 μL. Results Notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 had good linearity within the range of 0. 0300-0. 3000,0. 1201-1. 2009,0. 1200-1. 1997 μg respectively. The average recoveries（ n = 6） were 100. 43%,100. 65%,98. 74% and RSDs were 1. 51%,1. 30%,1. 03%,respectively. According to the content determination method for the determination of two Danqi tablets sample were similar. Conclusion UPLC method is more convenient,efficient,accurate and excellent repeatability,analysis is nearly eight times faster,can be used for determination of notoginsenosideR1,ginsenoside Rg1 and ginsenoside Rb1 in Danqi tablets.
出处
《中国生化药物杂志》
CAS
2015年第1期148-150,共3页
Chinese Journal of Biochemical Pharmaceutics
基金
国家自然科学基金(81271625)
