摘要
目的研究参菊洗剂的体外抑菌作用。方法采用琼脂扩散纸片法测定抑菌圈直径,采用液体培养液连续稀释法测定最低抑菌浓度(MIC),采用琼脂培养液平板法测定最低杀菌浓度(MBC),并与市售某妇科洗液比较抑菌作用。结果参菊洗剂对5种病原菌株的抑菌作用明显优于相同浓度的市售某妇科洗液,差异有统计学意义(P<0.05);参菊洗剂对金黄色葡萄球菌、铜绿假单胞菌、大肠埃希菌、枯草芽孢杆菌、白念珠菌的MIC分别为33.75,67.50,67.50,67.50,33.75 mg·m L-1;参菊洗剂对金黄色葡萄球菌、铜绿假单胞菌、大肠埃希菌、枯草芽孢杆菌、白念珠菌的MBC分别为33.75,67.50,67.50,135.00,33.75 mg·m L-1。结论参菊洗剂具有较明显的抑菌和杀菌作用。
Objective To study the antibacterial effect of Shenju lotion in vitro. Methods The diameter of inhibition zone was determined by paper-disc agar-diffusion method. Minimum inhibitory concentration （MIC） and Minimum bactericidal concentration （MBC） was determined by culture medium dilution methodand agar medium plate method, respectively.Antibacterial effect was compared between Shenju lotion and city sale of the gynecological lotion. Results Inhibitory effects of Shenju lotion on 5 pathogenic strains were significantly better than that of city sale of the gynecological lotion at the same concentrations （P〈0.05）. MIC of Shenju lotion on Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Bacillus subtilis and Candida albicans was 33.75, 67.50, 67.50, 67.50 and 33.75 mg·m L^-1 , respectively.MBC of Shenju lotion on Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Bacillus subtilis and Candida albicaus was 33.75, 67.50, 67.50, 135.00 and 33.75 mg·m L^-1, respectively. Conclusion Shenju lotion has obvious bacteriostasis and sterilization effect.
出处
《医药导报》
CAS
2015年第8期1032-1035,共4页
Herald of Medicine
基金
湖北省黄冈市科技计划项目(黄科字[2013]76号)
关键词
参菊洗剂
琼脂扩散纸片法
液体培养液连续稀释法
琼脂培养液平板法
抑菌作用
体外
Shenju lotion
Paper-disc agar-diffusion method
Liquid culture medium dilution method
Agar mediumplate method
Antibacterial effect, in vitro
