摘要
目的探讨德都红花-7味散对肝纤维化的治疗作用及其与基质金属蛋白酶(MMPs)表达及转化生长因子β1(TGF-β1)的相关性。方法大鼠分成4组,空白组、模型组、对照组、实验组,每组8只。用四氯化碳诱导建立大鼠肝纤维化模型。同时灌胃给药,对照组给予秋水仙碱片,用CMC纤维素钠水配制成2%的混悬液,每次0.2 m L·kg-1,1次/日;实验组给予德都红花-7味散,用CMC纤维素钠水配制成3%的混悬液,每次0.2 m L·kg-1;空白组和模型组给予等体积纯净水,持续40 d后,处死大鼠。用蛋白印迹法检测基质金属蛋白酶-1(MMP-1)、基质金属蛋白酶-13(MMP-13)、基质金属蛋白酶抑制剂-1(TIMP-1)蛋白;实时荧光定量PCR法检测Ⅰ型胶原mRNA、Ⅲ型胶原mRNA、TGF-β1mRNA的表达变化。结果模型组,MMP-1、MMP-13蛋白表达较空白组显著下调(P<0.05),TIMP-1蛋白表达较空白组显著上调(P<0.05);Ⅰ型胶原mRNA和Ⅲ型胶原mRNA、TGF-β1mRNA表达比空白组显著上调(P<0.05)。对照组和实验组,MMP-1、MMP-13蛋白表达较模型组显著上调(P<0.05),TIMP-1蛋白表达较模型组显著下调(P<0.05);Ⅰ型胶原mRNA和Ⅲ型胶原mRNA、TGF-β1mRNA表达较模型组显著下调(P<0.05)。结论德都红花-7味散通过调节和维持MMP/TIMP的稳态,阻断TGF-β表达和抑制Ⅰ型胶原mRNA和Ⅲ型胶原mRNA、TGF-β1mRNA的转录,达到延缓肝纤维化的发生发展。
Objective To observe the therapeutic effect of Deduhonghua - 7 powder on liver fibrosis and its correlation with matrix metailoproteinases （MMPs） and transforming growth factor β1 （ TGF - β1 ）. Methods The rats were divided into d groups, blank group, model group, control group, experiment group, 8 rats each group. The CCI4 was used to induce rat liver fibrosis. The control group was given colchicine pill, CMC sodium cellulosate was used to prepare 2% of colchicine suspension, once time per day, 0.2 mL · kg^-1 each time. The experiment group was given Deduhonghua -7 powder, CMC sodium cellulosate was used to prepare 3% of Deduhonghua -7 powder suspension, once time per day, 0. 2 mL · kg^-1 each time; blank group and model group were given same volume of pure water. All rats were sacrificed after 40 days of medication. Western blot was acted to detect the matrix metalloproteinase -1 （ MMP - 1 ）, matrix metalloproteinase - 13 （ MMP - 13 ）, tissue inhibitor of metalloproteinases - 1 （ TIMP - 1 ） protein expression. The PCR was used to detect the changesoftype I , llI collagen, TGF - β1 mRNA gene expression. Results The MMP - 1, MMP - 13 protein expression in rats of model group was down - regulated compared with blank group （ P 〈 0. 05 ）. The TIMP - 1 protein expression was up - regulated compared with blank group （ P 〈 0.05 ）. The mRNA expressions of type Ⅰ procollagen, type Ⅲ procollagen and TGF - β1 were increased compared with blank group（ P 〈 0.05 ）. The MMP - 1, MMP - 13 protein expression of control group and experiment group were significantly up - regulated compared with model group. The TIMP - 1 protein expression significantly down - regulated compared with model group. The mRNA gene expression of type Ⅰ procollagen, type Ⅲ procollagen and TGF - β1 were down - regulated compared with model group （ P 〈 0.05 ）. Conclusion Deduhonghua - 7 powder regulate and maintain the stabilization of MMP/TIMP to block the expression of TGF - 13 and inhibit the t
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2015年第15期1526-1529,共4页
The Chinese Journal of Clinical Pharmacology
基金
国家自然科学基金资助项目(81260574)
