摘要
目的:从SRA信号通路研究黄芩苷对乙酰低密度脂蛋白(Ac-LDL)+脂多糖(LPS)诱导的与动脉粥样硬化(AS)相关的巨噬细胞凋亡的影响。方法:收集鼠腹腔巨噬细胞进行体外培养,使用100μg/m L Ac-LDL及1μg/m L LPS诱导巨噬细胞凋亡,以120、240、480μg/m L黄芩苷作为低、中、高3种剂量进行干预,Annexin VFITC和PI双染后用流式细胞仪检测细胞凋亡,Western Blot技术检测SRA蛋白表达量。结果:与正常组比较,对照组与模型组的细胞凋亡率差异有统计学意义(P<0.01);低剂量观察组与模型组细胞凋亡率差异无统计学意义;中、高剂量观察组与模型组相比细胞凋亡率差异有统计学意义(P<0.01)。LPS对照组较Ac-LDL对照组的SRA含量显著增高(P<0.05),与黄芩苷低、中、高剂量组相比SRA表达量无统计学差异。结论:SRA信号通路参与了LPS和Ac-LDL双重因素诱导的巨噬细胞凋亡,并且SRA通路蛋白的激活主要与LPS干预相关。
Objective: To study the effects of baicalin on the macrophage apoptosis regarding atherosclerosis (AS) induced by acetyl low-density lipoprotein (Ac-LDL) and lipopolysaccharide (LPS) from the SRA signal pathway. Methods: The mouse peritoneal macrophages were cultured in vitro, 100μg/mL of Ac-LDL and 1μg/mL of LPS were used to induce macrophage apoptosis, and 120, 240 and 480μg/mL of baicalin as low, medium and high dose were used for intervention. AnnexinV-FITC and PI detected apoptosis by flow cytometer after staining; Western Blot was used to detect SRA protein expression. Results: Compared with the normal group, apoptosis rate had significant difference in the control groups and model group (P〈0.01). The apoptosis rate in the low-dose observation group had no significant difference with the model group, while that in the medium and high-dose observation group had significant difference (P〈0.01). The content in the LPS control group was higher than that in the Ac-LDL control group (P〈0.05), however, there was no significant difference when compared the low, medium and high-dose observation group. Conclusion: The SRA signal pathway participates in the macrophage apoptosis induced by LPS and Ac-LDL, moreover, the activation of proteins in SRA pathway is mainly associated with LPS.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2015年第8期3014-3017,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
广东省中医药管理局科研课题(No.20151196)~~
关键词
脂多糖
乙酰低密度脂蛋白
SRA
巨噬细胞
凋亡
Lipopolysaccharide
Acetyl low-density lipoprotein
SRA
Macrophage
Apoptosis