摘要
【目的】探讨肺炎衣原体(Chlamydia pneumoniae,CPn)感染对血清炎症因子水平与主动脉粥样硬化斑块面积的影响及黄芩苷的治疗作用。【方法】C57BL/6J小鼠随机分为黄芩苷高剂量组(剂量为50 mg.kg-1.d-1)、黄芩苷低剂量组(剂量为25 mg.kg-1.d-1)、阿奇霉毒组(剂量为5 mg.kg-1.d-1)、模型组和正常对照组。除正常对照组外,其他组均在喂饲高胆固醇饲料1周后,经一侧鼻腔吸入含CPn的培养液,每周1次,共3次;最后1次接种5 d后开始给药至实验结束,第1次接种后至第18周时处死全部动物,采用酶联免疫吸附法检测血清肿瘤坏死因子(TNF-α)、白细胞介素(IL-6、IL-10)的水平,及主动脉斑块面积指数(IPA)。【结果】模型组的血清TNF-α、IL-6、IL-10水平和IPA均高于正常对照组(P<0.01)。黄芩苷高剂量组和黄芩苷低剂量组的以上4个指标均比模型组低(均P<0.01),其中血清IL-10接近正常对照组水平(P>0.05);而阿奇霉素组的血清TNF-α、IL-6水平也比模型组低(均P<0.01),但血清IL-10与模型组比较无显著性变化(P>0.05)。3个治疗组均可改善模型动物的主动脉粥样硬化斑块,但黄芩苷2个治疗组的硬化斑块比阿奇霉素组小,黄芩苷低剂量组的IPA与阿奇霉素组相比,差异具有统计学意义(P<0.05)。【结论】早期给予高、低剂量黄芩苷或阿奇霉素治疗可不同程度降低高胆固醇合CPn感染引起的炎症反应,并有助于减轻CPn感染的高胆固醇饮食小鼠主动脉粥样硬化斑块损害程度;而黄芩苷的作用比阿奇霉素略优。
[Objective] To observe the influence of chlamydia pneumoniae infection on sermn inflammatory factors and atherosclerotic plaque area, and to investigate the therapeutic effect of baicalin. [Methods] C57BL/6J mice were randomized into 6 groups： normal, model, azithromycin （5 mg· kg^-1· d^-1 ） and baicalin groups （50 and 25 mg· kg^-1· d^-1 respectively）. Except mice in the normal group, the other mice were fed with forage containing high cholesterol for one week and then were inoculated by inhalation of chlamydia pneumoniae culture via nasopharynx weekly for three times （one time per week）. Drug administration started 5 days after the last inoculation and ended in 18 weeks after the first inoculation. After treatment, the mice were executed, the serum tumor necrosis factor α （TNF-α）, interleukin-6 （IL- 6）, IL-10 levels were examined by enzyme immunosorbent assay （ELISA） and aortic atherosclerotic plaque area index （IPA） was also detected. [Results] Sermn TNF-α, IL-6, IL-10 levels and /PA in the model group were higher than those in the normal group （ P 〈 0.01 ）, and were decreased in the high and low-dose baicalin groups （ P 〈 0.01 as compared with the model group） and IL-10 level was near to that in the normal group （ P 〉 0.05）. Serum TNF-α and IL-6 levels in azithromycin group were lower than those in the model group （ P 〈 0.01 ） while IL-10 level was similar to that in the model group （ P 〉 0.05）. IPA Was decreased in baicalin groups and azithromycin group, particularly in baicalin groups, and the difference was significant between low-dose baicalin group and azithromycin group （P 〈 0.05）. [ Conclusion ] Early treatment of baicalin or azithromycin can relieve the inflammatory reaction induced by cIflamydia pneumoniae infection and high cholesterol diet, and lessen aortic atherosclerotic plaque lesions in mice. And the effect of baicalin is better than that of azithromycin.
出处
《广州中医药大学学报》
CAS
2006年第4期322-324,329,共4页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(编号:30070938)
