摘要
目的观察摄食地沟油对小鼠肝肾功能及脂肪代谢的影响。方法将72只小鼠平均分为6组,其中地沟油组与食用油组各3组,按照5、10和20μL/g剂量的地沟油或食用油连续隔天给予小鼠灌胃8周,比较两组小鼠灌胃前后丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)、血尿素氮(BUN)、血肌酐(Scr)、甘油三酯(TG)和总胆固醇(TC)水平。结果地沟油的水分含量、酸价和过氧化值指标均超标。连续灌胃8周后,5μL/g剂量地沟油组小鼠ALT明显高于食用油组(P<0.05);10μL/g剂量地沟油组小鼠AST高于食用油组(P<0.05)。5、10和20μL/g剂量地沟油组小鼠Scr均高于同剂量食用油组(P<0.05);10和20μL/g剂量地沟油组小鼠BUN均高于同剂量食用油组(P<0.05);但相同剂量地沟油和食用油组小鼠的TG和TC未见统计学差异(P>0.05)。结论连续8周摄食地沟油对小鼠肝、肾功能均有明显损害作用。
Objective To determine the effects of gutter oil on liver function,renal function and serum lipid metabolism in mice.Methods A total of 72 mice were averagely divided into 6 groups.Mice were gavaged with different dosages （5 μL/g,1 0 μL/g and 20 μL/g）of gutter oil or cooking oil every other day for continuing 8 weeks.The indexes of liver, kidney and serum lipid parameters were assayed.Results The moisture,acid value and peroxide value of the gutter oil exceeded the national standard.After continuing 8 weeks＇intake of oils,alanine aminotransferase （ALT）of gutter oil group with a dosage of 5 μL/g,as well as aspartate aminotransferase （AST）of gutter oil group with a dosage of 1 0 μL/g significantly increased （P 〈0.05）,compared with the cooking oil group.Serum creatinine （Scr）of the gutter oil groups with 3 dosages significantly increased compared with the control groups （P 〈0.05 ）.Meanwhile,blood urea nitrogen （BUN）of gutter oil groups with a dosage of 5 μL/g and 1 0 μL/g were also significantly increased respectively（P 〈0.05）. However,no significant differences were observed in triglyceride （TG）and total cholesterol （TC）among the groups. Conclusion Eight weeks＇intake of gutter oil could cause the damage to liver and renal functions.
出处
《浙江预防医学》
2015年第8期764-767,共4页
Zhejiang Journal of Preventive Medicine
基金
浙江省大学生科技创新活动计划资助项目(2012R401148)
关键词
地沟油
肝功能
肾功能
血脂
Gutter oil
Liver function
Renal function
Serum lipid
