摘要
目的:观察甘草次酸(Glycyrrhetinic acid,GA)对哮喘大鼠肺泡灌洗液(Bronchoalveolar lavage fluid,BALF)白细胞计数及血清Ig E、IL-4、TNF-α水平的影响,探讨其防治哮喘的机制。方法:将60只雄性SD大鼠随机分为正常对照组、哮喘模型组、地塞米松0.5 mg/kg组、甘草次酸200、100、50 mg/kg剂量组。采用卵清白蛋白(OVA)与氢氧化铝致敏,并OVA雾化吸入激发制备大鼠支气管哮喘模型。各组给予相应剂量的药物7d,于末次给药后24h,处死动物,取血清测IgE、IL-4及TNF-α含量,收集BALF进行白细胞分类计数,Western-blot法检测甘草次酸对哮喘大鼠肺组织IgE、IL-4及TNF-α蛋白表达的影响。结果:与哮喘模型组比较,甘草次酸200、100 mg/kg可使哮喘大鼠BALF淋巴细胞及嗜酸性粒细胞数降低,中性粒细胞数增多,使血清及肺组织IgE、IL-4及TNF-α水平明显下降。结论:甘草次酸可通过减少炎性细胞(淋巴细胞及嗜酸性粒细胞)和下调细胞因子IgE、IL-4及TNF-α水平减轻哮喘大鼠气道炎症,改善哮喘症状。
Objective： To study the effects of glycyrrhetinic acid （GA） on broncholaveolar lavage fluid （BALF） leukocytes and serum IgE, IL-d and TNF-ct in bronchial asthma rats, and explore the anti-asthma mechanisms of GA. Methods： Sixty male Sprague-Dawley（SD） rats were randomly divided into 6 groups： blank control, asthma model, dexmnethasone （Dex） and GA 200, 100, 50 mg/kg group. Except those in the blank control group, all rats in other group were given intraperitoneal injection of ovalbumin （OVA） and aluminum hydroxide to sensitize, and then OVA was inhaled to stimulate to induce bronchial asthma. After modeling, the corresponding drugs were given the treatment groups ; the blank control group and model group were given normal saline （ NS）, once a day for 7 days. The rats were sacrificed after 24h in last
出处
《中药药理与临床》
CAS
CSCD
北大核心
2015年第3期52-55,共4页
Pharmacology and Clinics of Chinese Materia Medica
