摘要
目的：研究当归精油对实验性哮喘大鼠模型的平喘作用及对肺脏IL-10与Foxp3表达的影响。方法：取雌性大鼠随机分6组（n=9）,通过注射卵白蛋白（OVA）和氢氧化铝致敏与OVA雾化吸入激发复制哮喘模型,经当归精油干预,观测哮喘大鼠肺功能、哮喘行为学、支气管肺泡灌洗液（BALF）中IL-10（ELISA法）以及肺组织Foxp3（免疫组化法）的表达。结果：40~160 mg/kg当归精油可明显改善哮喘大鼠的肺功能,减轻吸入混合激发液后的哮喘等行为学症状,提高BALF中IL-10的水平及肺组织Foxp3蛋白的表达强度。结论：当归精油具有一定的平喘作用,而改善Treg细胞免疫功能异常低下是其作用机制之一。
Objective： To study the effects of Volatile Oil of Radix Angelicae Sinensis （VOA）on experimental asthma in rat model based on abnormal immune functions of Treg cells. Methods： After grouping, the asthmatic rats were developed through injecting OVA and AI（OH）3 for sensitization and then administering OVA aerosol for challenge, and the respiratory functions, asthmatic behaviors, IL-IO levels in broncho- alveolar lavage fluid（BALF） （ELISA） and Foxp3 expression （immunohistochemistry） in lung of asthmatic rats were observed. Rmdts： VOA at the doses of 40 ~ 160 mg/kg could improve the respiratory functions and the asthmatic behaviors, and upgrade IL-10 levels in BALF and Foxp3 expression in lung of asthmatic rats. Conclusion： VOA has some effects of anti-asthma and one of the mechanisms is to improving the lower immune functions of Treg cells.
出处
《中国应用生理学杂志》
CAS
CSCD
2015年第2期107-110,共4页
Chinese Journal of Applied Physiology
基金
国家自然科学基金项目(81460668)
甘肃省自然科学基金项目(1310RJZA086)
甘肃省中药药理与毒理学重点实验室开放基金项目(ZDSYS-KJ-2013-008)
