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抑黑素对癫痫模型大鼠海马区GluR2表达及认知功能的影响 被引量:2

Effects of melatonin on expression of GluR2 in the hippocampus and cognitive function of rat models of epilepsy
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摘要 背景:目前研究数据显示癫痫患者常伴有认知障碍等并发症,近年来研究表明抑黑素对癫痫起抑制作用,但其机制不明。目的:探讨抑黑素对癫痫模型大鼠认知功能及海马区GluR 2表达的影响,进而研究抑黑素抗癫痫的作用机制。方法:采用氯化锂-皮罗卡品诱导构建慢性癫痫大鼠模型,分别腹腔注射适量的生理盐水和抑黑素进行对比观察,并设置对照组。结果与结论:造模后第4,6周,癫痫+抑黑素组大鼠海马区GluR 2表达量高于癫痫组(P<0.05);造模后第4,6周,对照组及癫痫+抑黑素组大鼠海马CA1区突触膜蛋白中GluR 2的表达量高于癫痫组(P<0.05);造模5 d后,与癫痫组和癫痫+生理盐水组相比,癫痫+抑黑素组大鼠的逃避潜伏期、运行时间、主动回避潜伏期、被动回避潜伏期都显著降低(P<0.05),正确率和主动回避次数显著增高(P<0.05)。结果证实,抑黑素能够通过上调癫痫模型大鼠海马CA1区突触膜上GluR 2的表达来改善其认知功能损害。 BACKGROUND: Current research data have shown that patients with epilepsy are often accompanied by complications such as cognitive impairment. Recent studies have demonstrated that melatonin has an inhibitory effect on epilepsy, but its underlying mechanism is unknown. OBJECTIVE: To investigate the effects of melatonin on the cognitive function and Glu R2 expression in the hippocampus of rat models of epilepsy, and further study the mechanism of melatonin against epilepsy. METHODS: Rat models of chronic epilepsy were established by intraperitoneal injection of lithium chloride-pilocarpine, and intraperitoneally injected with sufficient amount of physiological saline and melatonin respectively. Control group was set for observation. RESULTS AND CONCLUSION: At 4 and 6 weeks after modeling, the Glu R2 expression level in the hippocampus of rats in the epilepsy + melatonin group was significantly higher than that in the epilepsy group(P〈0.05); the Glu R2 expression level in the synaptic membrane of hippocampal CA1 region of rats in the control and epilepsy + melatonin groups was significantly higher than that in the epilepsy group(P〈0.05). At 4 days after modeling, compared with epilepsy group and epilepsy + physiological saline group, the escape latency, operation time, active avoidance latency, passive avoidance latency of rats in the epilepsy + melatonin group were significantly decreased(P〈0.05), the correct rate and active avoidance number were significantly increased(P〈0.05). These results demonstrate that melatonin can improve the cognitive function of rat models of epilepsy by up-regulating the expression of Glu R2 in the synaptic membrane of hippocampal CA1 region.
出处 《中国组织工程研究》 CAS 北大核心 2015年第27期4328-4332,共5页 Chinese Journal of Tissue Engineering Research
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